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Identification of transcriptional signatures for cell types from single-cell RNA-Seq

Ntranos, Vasilis and Yi, Lynn and Melsted, Páll and Pachter, Lior (2018) Identification of transcriptional signatures for cell types from single-cell RNA-Seq. . (Unpublished) http://resolver.caltech.edu/CaltechAUTHORS:20181031-090523070

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Abstract

Single-cell RNA-Seq makes it possible to characterize the transcriptomes of cell types and identify their transcriptional signatures via differential analysis. We present a fast and accurate method for discriminating cell types that takes advantage of the large numbers of cells that are assayed. When applied to transcript compatibility counts obtained via pseudoalignment, our approach provides a quantification-free analysis of 3' single-cell RNA-Seq that can identify previously undetectable marker genes.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/258566DOIDiscussion Paper
ORCID:
AuthorORCID
Yi, Lynn0000-0003-4575-0158
Melsted, Páll0000-0002-8418-6724
Pachter, Lior0000-0002-9164-6231
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. bioRxiv preprint first posted online Feb. 1, 2018. We thank Nicolas Bray, Jase Gehring and Valentine Svensson for discussion and comments on the manuscript. Harold Pimentel assisted with the simulations. We thank Charlotte Soneson and Mark Robinson for questions that led to the clarification of GDE, DGE, DTU and DTE in Supplementary Figure 1.
Record Number:CaltechAUTHORS:20181031-090523070
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20181031-090523070
Official Citation:Identification of transcriptional signatures for cell types from single-cell RNA-Seq. Vasilis Ntranos, Lynn Yi, Pall Melsted, Lior Pachter. bioRxiv 258566; doi: https://doi.org/10.1101/258566
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90536
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 Nov 2018 17:56
Last Modified:01 Nov 2018 17:56

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