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Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the drosophila germ line

Goldberg, David A. and Posakony, James W. and Maniatis, Tom (1983) Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the drosophila germ line. Cell, 34 (1). pp. 59-73. ISSN 0092-8674. https://resolver.caltech.edu/CaltechAUTHORS:20181031-133250264

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Abstract

We have used P-element-mediated transformation to introduce a cloned Drosophila alcohol dehydrogenase (Adh) gene into the germ line of ADH null flies. Six independent transformants expressing ADH were identified by their acquired resistance to ethanol. Each transformant carries a single copy of the cloned Adh gene in a different chromosomal location. Four of the six transformant lines exhibit normal Adh expression by the following criteria: quantitative levels of ADH enzyme activity in larvae and adults; qualitative tissue specificity; the size of stable Adh mRNA; and the characteristic developmental switch in utilization of two different Adh promoters. The remaining two transformants express ADH enzyme activity with the correct tissue specificity, but at a lower level than wild type. These results demonstrate that an 11.8 kb chromosomal fragment containing the Adh gene includes the cis-acting sequences necessary for its correct developmental expression, and that a variety of chromosomal sites permit proper Adh gene function.


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https://doi.org/10.1016/0092-8674(83)90136-8DOIArticle
Additional Information:© 1983 by MIT. Received 27 May 1983. We wish to acknowledge the excellent technical assistance of Karen Livingstone and Abby Telfer. Tip Benyajati, Nick Spoerel, and Kevin O'Hare generously provided unpublished data. We thank Gerry Rubin and Allan Spradling for providing P element vectors, Bill Sofer for fly stocks, and Nick Spoerel for plasmid subclones. J. W. P. was supported by an American Cancer Society postdoctoral fellowship. This work was funded by a grant from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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American Cancer SocietyUNSPECIFIED
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Issue or Number:1
Record Number:CaltechAUTHORS:20181031-133250264
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181031-133250264
Official Citation:David A. Goldberg, James W. Posakony, Tom Maniatis, Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the drosophila germ line, Cell, Volume 34, Issue 1, 1983, Pages 59-73, ISSN 0092-8674, https://doi.org/10.1016/0092-8674(83)90136-8. (http://www.sciencedirect.com/science/article/pii/0092867483901368)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90549
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 Nov 2018 17:43
Last Modified:03 Oct 2019 20:26

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