Dual-view photoacoustic microscopy for quantitative cell nuclear imaging

Cai, De and Wong, Terence T. W. and Zhu, Liren and Shi, Junhui and Chen, Sung-Liang and Wang, Lihong V. (2018) Dual-view photoacoustic microscopy for quantitative cell nuclear imaging. Optics Letters, 43 (20). pp. 4875-4878. ISSN 0146-9592. PMCID PMC6245540. https://resolver.caltech.edu/CaltechAUTHORS:20181101-114159377

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Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging imaging modality for studying biological tissues. However, in conventional single-view OR-PAM, the lateral and axial resolutions—determined optically and acoustically, respectively—are highly anisotropic. In this Letter, we introduce dual-view OR-PAM to improve axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 μm polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis.

Item Type:

Article

Related URLs:

URL	URL Type	Description
https://doi.org/10.1364/ol.43.004875	DOI	Article
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245540	PubMed Central	Article

ORCID:

Author	ORCID
Wong, Terence T. W.	0000-0001-6399-758X
Zhu, Liren	0000-0002-6338-9338
Shi, Junhui	0000-0002-5741-2781
Chen, Sung-Liang	0000-0002-0572-5110
Wang, Lihong V.	0000-0001-9783-4383

Additional Information:

© 2018 Optical Society of America. Received 25 July 2018; accepted 31 August 2018; posted 10 September 2018 (Doc. ID 340705); published 3 October 2018. D. Cai appreciates the support from China Scholarship Council. L. V. Wang has a financial interest in Microphotoacoustics, Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work. Funding: National Institutes of Health (NIH) (DP1 EB016986, R01 CA186567, U01 NS090579, U01 NS099717).

Funders:

Funding Agency	Grant Number
NIH	DP1 EB016986
NIH	R01 CA186567
NIH	U01 NS090579
NIH	U01 NS099717
China Scholarship Council	UNSPECIFIED

Issue or Number:

Classification Code:

OCIS codes: (110.0180) Microscopy; (110.5120) Photoacoustic imaging; (170.3880) Medical and biological imaging; (100.6890) Three-dimensional image processing

PubMed Central ID:

PMC6245540

Record Number:

CaltechAUTHORS:20181101-114159377

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20181101-114159377

Official Citation:

De Cai, Terence T. W. Wong, Liren Zhu, Junhui Shi, Sung-Liang Chen, and Lihong V. Wang, "Dual-view photoacoustic microscopy for quantitative cell nuclear imaging," Opt. Lett. 43, 4875-4878 (2018)

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No commercial reproduction, distribution, display or performance rights in this work are provided.

ID Code:

90563

Collection:

CaltechAUTHORS

Deposited By:

George Porter

Deposited On:

01 Nov 2018 21:28

Last Modified:

07 Apr 2020 15:43

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