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Dual-view photoacoustic microscopy for quantitative cell nuclear imaging

Cai, De and Wong, Terence T. W. and Zhu, Liren and Shi, Junhui and Chen, Sung-Liang and Wang, Lihong V. (2018) Dual-view photoacoustic microscopy for quantitative cell nuclear imaging. Optics Letters, 43 (20). pp. 4875-4878. ISSN 0146-9592. PMCID PMC6245540. doi:10.1364/ol.43.004875.

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Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging imaging modality for studying biological tissues. However, in conventional single-view OR-PAM, the lateral and axial resolutions—determined optically and acoustically, respectively—are highly anisotropic. In this Letter, we introduce dual-view OR-PAM to improve axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 μm polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Wong, Terence T. W.0000-0001-6399-758X
Zhu, Liren0000-0002-6338-9338
Shi, Junhui0000-0002-5741-2781
Chen, Sung-Liang0000-0002-0572-5110
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2018 Optical Society of America. Received 25 July 2018; accepted 31 August 2018; posted 10 September 2018 (Doc. ID 340705); published 3 October 2018. D. Cai appreciates the support from China Scholarship Council. L. V. Wang has a financial interest in Microphotoacoustics, Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work. Funding: National Institutes of Health (NIH) (DP1 EB016986, R01 CA186567, U01 NS090579, U01 NS099717).
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIHU01 NS090579
NIHU01 NS099717
China Scholarship CouncilUNSPECIFIED
Issue or Number:20
Classification Code:OCIS codes: (110.0180) Microscopy; (110.5120) Photoacoustic imaging; (170.3880) Medical and biological imaging; (100.6890) Three-dimensional image processing
PubMed Central ID:PMC6245540
Record Number:CaltechAUTHORS:20181101-114159377
Persistent URL:
Official Citation:De Cai, Terence T. W. Wong, Liren Zhu, Junhui Shi, Sung-Liang Chen, and Lihong V. Wang, "Dual-view photoacoustic microscopy for quantitative cell nuclear imaging," Opt. Lett. 43, 4875-4878 (2018)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90563
Deposited By: George Porter
Deposited On:01 Nov 2018 21:28
Last Modified:16 Nov 2021 03:33

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