van den Berg, Bert and Black, Paul N. and Clemons, William M., Jr. and Rapoport, Tom A. (2004) Crystal Structure of the Long-Chain Fatty Acid Transporter FadL. Science, 304 (5676). pp. 1506-1509. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20181101-114159594
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Abstract
The mechanisms by which hydrophobic molecules, such as long-chain fatty acids, enter cells are poorly understood. In Gram-negative bacteria, the lipopolysaccharide layer in the outer membrane is an efficient barrier for fatty acids and aromatic hydrocarbons destined for biodegradation. We report crystal structures of the long-chain fatty acid transporter FadL from Escherichia coli at 2.6 and 2.8 angstrom resolution. FadL forms a 14-stranded β barrel that is occluded by a central hatch domain. The structures suggest that hydrophobic compounds bind to multiple sites in FadL and use a transport mechanism that involves spontaneous conformational changes in the hatch.
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| Additional Information: | © 2004 American Association for the Advancement of Science. 4 March 2004; accepted 4 May 2004. We would like to thank M. Becker and L. Berman for support at beamline X25 at the National Synchrotron Light Source (Brookhaven National Laboratory), and C. Ogata and M. Capel for help at beamline 8BM at the Advanced Photon Source (Northeastern Collaborative Access team). We also would like to thank S. C. Harrison for in-house x-ray generator access and L. S. Wolff for performing fatty acid transport and binding experiments. P.N.B. received support from the NSF. W.M.C. is supported by a fellowship from the Damon Runyon Cancer Research Foundation. T.A.R is a Howard Hughes Medical Institute investigator. Coordinates and structure factors have been deposited in the Protein Data Bank with accession codes 1T16 (monoclinic crystal form) and 1T1L (hexagonal crystal form). | |||||||||
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| Issue or Number: | 5676 | |||||||||
| Record Number: | CaltechAUTHORS:20181101-114159594 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20181101-114159594 | |||||||||
| Official Citation: | Crystal Structure of the Long-Chain Fatty Acid Transporter FadL BY BERT VAN DEN BERG, PAUL N. BLACK, WILLIAM M. CLEMONS JR., TOM A. RAPOPORT Science 04 Jun 2004: Vol. 304, Issue 5676, pp. 1506-1509 DOI: 10.1126/science.1097524 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 90564 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 01 Nov 2018 19:59 | |||||||||
| Last Modified: | 03 Oct 2019 20:26 |
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