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Whole-Brain Analysis of Cells and Circuits by Tissue Clearing and Light-Sheet Microscopy

Mano, Tomoyuki and Albanese, Alexandre and Dodt, Hans-Ulrich and Erturk, Ali and Gradinaru, Viviana and Treweek, Jennifer B. and Miyawaki, Atsushi and Chung, Kwanghun and Ueda, Hiroki R. (2018) Whole-Brain Analysis of Cells and Circuits by Tissue Clearing and Light-Sheet Microscopy. Journal of Neuroscience, 38 (44). pp. 9330-9337. ISSN 0270-6474. PMCID PMC6706004. doi:10.1523/jneurosci.1677-18.2018.

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In this photo essay, we present a sampling of technologies from laboratories at the forefront of whole-brain clearing and imaging for high-resolution analysis of cell populations and neuronal circuits. The data presented here were provided for the eponymous Mini-Symposium presented at the Society for Neuroscience's 2018 annual meeting.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Albanese, Alexandre0000-0001-7093-574X
Dodt, Hans-Ulrich0000-0002-9784-9689
Erturk, Ali0000-0001-5163-5100
Gradinaru, Viviana0000-0001-5868-348X
Chung, Kwanghun0000-0002-8167-3340
Ueda, Hiroki R.0000-0001-8342-9176
Additional Information:© 2018 the authors. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license ( Received Aug. 31, 2018; revised Sept. 27, 2018; accepted Sept. 27, 2018. The Dodt laboratory thanks Dr. Saiedeh Saghafi and Dr. Klaus Becker for aspheric optics-based LSFM work, and the Austrian science funding agency FWF and the German Hertie foundation for support; the Ertürk laboratory thanks Ruiyao Cai for uDISCO figure and Synergy Excellence Cluster Munich (SyNergy), Fritz Thyssen Stiftung, and DFG for support; the Miyawaki laboratory thanks Dr. Hiroshi Hama for ScaleS figure and Grant-in-Aid for Scientific Research on Priority Areas (JSPS KAKENHI), the Human Frontier Science Program, and the Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) for support; the Ueda laboratory thanks Tatsuya Murakami for offering picture materials for CUBIC figure, and Brain/MINDS (AMED/MEXT), the Basic Science and Platform Technology Program for Innovative Biological Medicine (AMED/MEXT)and Grant-in-Aid for Scientific Research (S) (JSPS KAKENHI) for support; the Chung laboratory thanks The Packard Award, the McKnight Foundation and the NIH (1-DP2-ES027992) for support; the Gradinaru laboratory thanks Sripriya Ravindra Kumar, Gerard M. Coughlin, Rosemary Challis, and Collin Challis for CREATE and VAST images, Min Jee Jang for HCR images, and J. Ryan Cho for GCaMP imaging, and the NIH BRAIN Initiative and NIH Director's Office and NSF Neuronex for support. The authors declare no competing financial interests.
Funding AgencyGrant Number
FWF Der WissenschaftsfondsUNSPECIFIED
Hertie FoundationUNSPECIFIED
Synergy Excellence ClusterUNSPECIFIED
Fritz Thyssen StiftungUNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Japan Society for the Promotion of Science (JSPS)UNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
Brain Mapping by Integrated Neurotechnologies for Disease StudiesUNSPECIFIED
Ministry of Education, Culture, Sports, Science and Technology (MEXT)UNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
McKnight FoundationUNSPECIFIED
Issue or Number:44
PubMed Central ID:PMC6706004
Record Number:CaltechAUTHORS:20181105-084438080
Persistent URL:
Official Citation:Whole-Brain Analysis of Cells and Circuits by Tissue Clearing and Light-Sheet Microscopy. Tomoyuki Mano, Alexandre Albanese, Hans-Ulrich Dodt, Ali Erturk, Viviana Gradinaru, Jennifer B. Treweek, Atsushi Miyawaki, Kwanghun Chung, Hiroki R. Ueda. Journal of Neuroscience 31 October 2018, 38 (44) 9330-9337; DOI: 10.1523/JNEUROSCI.1677-18.2018
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90628
Deposited By: Tony Diaz
Deposited On:06 Nov 2018 17:43
Last Modified:16 Nov 2021 03:34

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