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Design Guidelines For Sequestration Feedback Networks

Baetica, Ania-Ariadna and Leong, Yoke Peng and Olsman, Noah and Murray, Richard M. (2018) Design Guidelines For Sequestration Feedback Networks. . (Unpublished)

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Integral control is commonly used in mechanical and electrical systems to ensure perfect adaptation. A proposed design of integral control for synthetic biological systems employs the sequestration of two biochemical controller species. The unbound amount of controller species captures the integral of the error between the current and the desired state of the system. However, implementing integral control inside bacterial cells using sequestration feedback has been challenging due to the controller molecules being degraded and diluted. Furthermore, integral control can only be achieved under stability conditions that not all sequestration feedback networks fulfill. In this work, we give guidelines for ensuring stability and good performance (small steady-state error) in sequestration feedback networks. Our guidelines provide simple tuning options to obtain a flexible and practical biological implementation of sequestration feedback control. Using tools and metrics from control theory, we pave the path for the systematic design of synthetic biological systems.

Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription Paper
Baetica, Ania-Ariadna0000-0003-0421-8181
Leong, Yoke Peng0000-0001-8560-8856
Olsman, Noah0000-0002-4351-3880
Murray, Richard M.0000-0002-5785-7481
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. bioRxiv preprint first posted online Oct. 30, 2018. The authors would like to thank Michael W. Chevalier and Hana El-Samad for providing feedback on the manuscript. The project was sponsored by the Defense Advanced Research Projects Agency (Agreement HR0011-17-2-0008). The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred. Author Contributions: Conceptualization and Methodology, AAB, YPL, NO, and RMM; Formal Analysis, AAB, YPL, and NO; Software, AAB and YPL; Writing, AAB; Supervision and Funding, RMM. The authors declare no competing interests.
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)HR0011-17-2-0008
Record Number:CaltechAUTHORS:20181107-093406633
Persistent URL:
Official Citation:Design Guidelines For Sequestration Feedback Networks. Ania-Ariadna Baetica, Yoke Peng Leong, Noah Olsman, Richard M. Murray. bioRxiv 455493; doi:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90699
Deposited By: Tony Diaz
Deposited On:07 Nov 2018 17:50
Last Modified:16 Nov 2021 03:34

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