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Imaging of gene expression in vivo with photoacoustic tomography

Li, Li and Zemp, Roger J. and Lungu, Gina and Stoica, George and Wang, Lihong V. (2006) Imaging of gene expression in vivo with photoacoustic tomography. In: Photons Plus Ultrasound: Imaging and Sensing 2006: The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics. Proceedings of SPIE. No.6086. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 608608. ISBN 9780819461285. https://resolver.caltech.edu/CaltechAUTHORS:20181107-113009773

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Abstract

In the post-genomic era, there is an increasing interest in visualizing the expression of functional genes in vivo. With the assistance of the reporter gene technique, various imaging modalities have been adopted for this purpose. In vivo gene expression imaging promises to provide biologists with a powerful tool for deepening our understanding of developmental biology, expanding our knowledge of the genetic basis of disease, and advancing the development of medicine. In this paper, we demonstrate the feasibility of imaging gene expression with photoacoustic imaging, which offers unique absorption contrast with ultrasonic resolution in vivo. We mark tumors in rats with the lacZ reporter gene. The lacZ gene encodes an enzyme β-galactosidase, which yields a dark blue product when acting on a colorimetric assay called X-gal. Photoacoustic tomography at 650nm clearly visualizes the presence of this blue product. The spectroscopic method can also potentially improve specificity. Considering how many staining methods are used in traditional biology, we believe that photoacoustic techniques will revolutionize the field of molecular imaging. The further development of reporter gene systems with high absorbing products in the NIR region is needed.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.646322DOIArticle
ORCID:
AuthorORCID
Stoica, George0000-0001-8071-4828
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2006 Society of Photo-optical Instrumentation Engineers (SPIE). We are grateful to Sergio Similache, and Ovidiu Craciun for assistance with animal handling. This research is funded in part by the National Institutes of Health grants R01 N846214 (BRP) and R01 EB000712.
Funders:
Funding AgencyGrant Number
NIHR01 N846214
NIHR01 EB000712
Subject Keywords:photoacoustic, molecular imaging, gene expression, reporter gene, lacZ
Series Name:Proceedings of SPIE
Issue or Number:6086
Record Number:CaltechAUTHORS:20181107-113009773
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181107-113009773
Official Citation:Li Li, Roger J. Zemp, Gina Lungu, George Stoica, Lihong V. Wang, } "Imaging of gene expression in vivo with photoacoustic tomography", Proc. SPIE 6086, Photons Plus Ultrasound: Imaging and Sensing 2006: The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 608608 (6 March 2006); doi: 10.1117/12.646322; https://doi.org/10.1117/12.646322
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90715
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Nov 2018 20:49
Last Modified:03 Oct 2019 20:28

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