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Scanning apertureless fluorescence microscope

Lessard, Guillaume A. and Yang, T. J. and Barritault, Pierre and Quake, Stephen R. (1999) Scanning apertureless fluorescence microscope. In: Scanning and Force Microscopies for Biomedical Applications. Proceedings of SPIE. No.3607. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, pp. 158-165. ISBN 9780819430779.

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We describe a near-field apertureless fluorescence microscope, capable of imaging fluorescent latex beads with subwavelength precision. The instrument is based on a home- built tapping-mode atomic-force microscope, to which an inverted optical microscope was added. The fact that the wavelength of the fluorescence that we observe is different from the wavelength of the illumination allows for a relatively straightforward detection mechanism. Sample images are presented, along with evidence that the observe effect is of optical origin.

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Additional Information:© 1999 Society of Photo-optical Instrumentation Engineers (SPIE). This work was supported by the Keck Foundation. G. L. had partial financial support from the Fonds FCAR.
Funding AgencyGrant Number
W. M. Keck FoundationUNSPECIFIED
Formation des chercheurs et aide a la recherche-QuebecUNSPECIFIED
Subject Keywords:Near-field, optics, AFM, fluorescence, force, light, apertureless NSOM
Series Name:Proceedings of SPIE
Issue or Number:3607
Record Number:CaltechAUTHORS:20181109-093018344
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Official Citation:Guillaume A. Lessard, T. Jason Yang, Pierre Barritault, Stephen R. Quake, } "Scanning apertureless fluorescence microscope", Proc. SPIE 3607, Scanning and Force Microscopies for Biomedical Applications, (17 June 1999); doi: 10.1117/12.350630;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90788
Deposited By: Tony Diaz
Deposited On:14 Nov 2018 19:25
Last Modified:16 Nov 2021 03:35

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