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Portable cellphone based microbial pathogen detection system for water quality analysis

Huang, Xiao and Lin, Xingyu and Urmann, Katharina and Li, Lijie and Hoffmann, Michael (2018) Portable cellphone based microbial pathogen detection system for water quality analysis. In: 256th American Chemical Society National Meeting & Exposition, 19-23 August 2018, Boston, MA.

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We developed a digital nucleic acid amplification test (dNAAT) assay for the detection of indicator and pathogenic microorganisms at the point-of-sample collection. The system, named gLAMP, uses polyethylene glycol (PEG) hydrogel as reaction matrix and loop-mediated isothermal amplification (LAMP) technique for target DNA/RNA amplification (Fig. 1). The system achieved similar sensitivity to real-time PCR in virus quantification (Fig. 2), and demonstrated higher tolerance against inhibitors present in natural water samples (e.g., wastewater, stormwater and pond water). gLAMP is fast taking only 30 min from sample-to-answer compared to 2-4 h for real-time PCR and several days to weeks for traditional culture-based detection method. A smartphone based handheld incubation and detection device is under develop to facilitate the field scale application of the technol. Considering its simplicity, sensitivity, and rapidity, gLAMP holds great potential for microbial water quality anal. in some low-resource settings.

Item Type:Conference or Workshop Item (Paper)
Related URLs:
URLURL TypeDescription Website
Huang, Xiao0000-0002-3737-6939
Lin, Xingyu0000-0002-0950-0736
Li, Lijie0000-0002-7988-6208
Hoffmann, Michael0000-0001-6495-1946
Additional Information:© 2018 American Chemical Society.
Record Number:CaltechAUTHORS:20181109-100111142
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90792
Deposited By: Tony Diaz
Deposited On:13 Nov 2018 18:14
Last Modified:03 Mar 2020 13:01

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