CaltechAUTHORS
  A Caltech Library Service

Motion-sensitive 3-D optical coherence microscope operating at 1300 nm for the visualization of early frog development

Hoeling, Barbara M. and Feldman, Stephanie S. and Strenge, Daniel T. and Bernard, Aaron and Hogan, Emily R. and Petersen, Daniel C. and Fraser, Scott E. and Kee, Yun and Tyszka, J. Michael and Haskell, Richard C. (2007) Motion-sensitive 3-D optical coherence microscope operating at 1300 nm for the visualization of early frog development. In: Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI. Proceedings of SPIE. No.6429. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 64292T. ISBN 9780819465429. http://resolver.caltech.edu/CaltechAUTHORS:20181109-133622413

[img] PDF - Published Version
See Usage Policy.

413Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20181109-133622413

Abstract

We present 3-dimensional volume-rendered in vivo images of developing embryos of the African clawed frog Xenopus laevis taken with our new en-face-scanning, focus-tracking OCM system at 1300 nm wavelength. Compared to our older instrument which operates at 850 nm, we measure a decrease in the attenuation coefficient by 33%, leading to a substantial improvement in depth penetration. Both instruments have motion-sensitivity capability. By evaluating the fast Fourier transform of the fringe signal, we can produce simultaneously images displaying the fringe amplitude of the backscattered light and images showing the random Brownian motion of the scatterers. We present time-lapse movies of frog gastrulation, an early event during vertebrate embryonic development in which cell movements result in the formation of three distinct layers that later give rise to the major organ systems. We show that the motion-sensitive images reveal features of the different tissue types that are not discernible in the fringe amplitude images. In particular, we observe strong diffusive motion in the vegetal (bottom) part of the frog embryo which we attribute to the Brownian motion of the yolk platelets in the endoderm.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.701420DOIArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5739-4026
Tyszka, J. Michael0000-0001-9342-9014
Additional Information:© 2007 Society of Photo-optical Instrumentation Engineers (SPIE).
Record Number:CaltechAUTHORS:20181109-133622413
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20181109-133622413
Official Citation:Barbara M. Hoeling, Stephanie S. Feldman, Daniel T. Strenge, Aaron Bernard, Emily R. Hogan, Daniel C. Petersen, Scott E. Fraser, Yun Kee, J. Michael Tyszka, Richard C. Haskell,} "Motion-sensitive 3-D optical coherence microscope operating at 1300 nm for the visualization of early frog development", Proc. SPIE 6429, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI, 64292T (7 February 2007); doi: 10.1117/12.701420; https://doi.org/10.1117/12.701420
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90802
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Nov 2018 00:41
Last Modified:14 Nov 2018 00:41

Repository Staff Only: item control page