A Caltech Library Service

Electrochemical cell lysis of Gram-positive and -negative bacteria for DNA extraction

Wang, Siwen and Hoffmann, Michael (2018) Electrochemical cell lysis of Gram-positive and -negative bacteria for DNA extraction. In: 256th American Chemical Society National Meeting & Exposition, 19-23 August 2018, Boston, MA.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


DNA extn. is an essential step for the nucleic acid-based microbial monitoring method. Chem. lysis is currently the most common technique for DNA extn. However, this method need time-consuming reagent adding process and residue removal step in the end to avoid the interference with the downstream detection. Elec. lysis based on irreversible electroporation has been reported for successful cell lysis with fast speed in several studies. But it requires a high voltage to achieve the crit. transmembrane potential and the joule heat due to the high voltage also affects the working fluid. In this study, a rapid, cost-effective and low power DNA extn. method without reagent adding process was developed based on electrochem. lysis. In the electrochem. reaction, hydroxide ions generated locally at cathode can break the fatty acid-glycerol ester bonds of phospholipid mols. within cell membrane and thereby release the intracellular materials. A cation exchange membrane was added between anodic and cathodic chambers for a fast accumulation of hydroxide ions. Fluorescent microscope and real-time PCR were used to measure the cell lysis efficiency and DNA extn. efficiency, resp. Both Gram-pos. bacteria (Enterococcus faecalis and Bacillus subtilis) and Gram-neg. bacteria (Escherichia coli, Salmonella typhi and Enterobacter) were successfully lysed within 1 min using 40 mA of DC (5 V). The DNA extn. efficiencies of Gram-neg. bacteria were over 80% and of Gram-pos. bacteria were 20%. A 3D-printed portable electrochem. cell lysis device was also developed for on-site field study and potentially could be a component for an integrated pathogen detection platform.

Item Type:Conference or Workshop Item (Paper)
Related URLs:
URLURL TypeDescription Website
Wang, Siwen0000-0002-8553-425X
Hoffmann, Michael0000-0001-6495-1946
Additional Information:© 2018 American Chemical Society.
Record Number:CaltechAUTHORS:20181109-161828042
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90816
Deposited By: Tony Diaz
Deposited On:13 Nov 2018 21:07
Last Modified:03 Mar 2020 13:01

Repository Staff Only: item control page