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A long-term depression of AMPA currents in cultured cerebellar purkinje neurons

Linden, David J. and Dickinson, Michael H. and Smeyne, Michelle and Connor, John A. (1991) A long-term depression of AMPA currents in cultured cerebellar purkinje neurons. Neuron, 7 (1). pp. 81-89. ISSN 0896-6273. https://resolver.caltech.edu/CaltechAUTHORS:20181116-074056175

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Abstract

Cerebellar long-term depression (LTD) is a model of synaptic plasticity in which conjunctive stimulation of parallel fiber and climbing fiber inputs to a Purkinje neuron induces a persistent depression of the parallel fiber-Purkinje neuron synapse. We report that an analogous phenomenon may be elicited in the cultured mouse Purkinje neuron when iontophoretic glutamate application and depolarization of the Purkinje neurons are substituted for parallel fiber and climbing fiber stimulation, respectively. The induction of LTD in these cerebellar cultures requires activation of both ionotropic (AMPA) and metabotropic quisqualate receptors, together with depolarization in the presence of external Ca^(2+). This postsynaptic alteration is manifest as a depression of glutamate or AMPA currents, but not aspartate or NMDA currents. These results strengthen the contention that the expression of cerebellar LTD is at least in part postsynaptic and provide evidence that activation of both ionotropic and metabotropic quisqualate receptors are necessary for LTD induction.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0896-6273(91)90076-CDOIArticle
ORCID:
AuthorORCID
Dickinson, Michael H.0000-0002-8587-9936
Additional Information:© 1991 by Cell Press. Received March 5, 1991; revised April 9, 1991. The authors gratefully acknowledge the assistance of Dr. Karl Schilling and Dr. James Morgan in tissue culture methodology and for providing the photograph of the PEP-19 stained PNs. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC Section 1734 solely to indicate this fact.
Issue or Number:1
Record Number:CaltechAUTHORS:20181116-074056175
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181116-074056175
Official Citation:David J. Linden, Michael H. Dickinson, Michelle Smeyne, John A. Connor, A long-term depression of AMPA currents in cultured cerebellar purkinje neurons, Neuron, Volume 7, Issue 1, 1991, Pages 81-89, ISSN 0896-6273, https://doi.org/10.1016/0896-6273(91)90076-C. (http://www.sciencedirect.com/science/article/pii/089662739190076C)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:90940
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Nov 2018 19:46
Last Modified:03 Oct 2019 20:30

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