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Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling

Hu, Fanghao and Wei, Lu and Zheng, Chaogu and Shen, Yihui and Min, Wei (2014) Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling. Analyst, 139 (10). pp. 2312-2317. ISSN 0003-2654. PMCID PMC4069604. https://resolver.caltech.edu/CaltechAUTHORS:20181119-102039701

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Abstract

Choline is a small molecule that occupies a key position in the biochemistry of all living organisms. Recent studies have strongly implicated choline metabolites in cancer, atherosclerosis and nervous system development. To detect choline and its metabolites, existing physical methods such as magnetic resonance spectroscopy and positron emission tomography are often limited by the poor spatial resolution and substantial radiation dose. Fluorescence imaging, although with submicrometer resolution, requires introduction of bulky fluorophores and thus is difficult in labeling the small choline molecule. By combining the emerging bond-selective stimulated Raman scattering microscopy with metabolic incorporation of deuterated choline, herein we have achieved high resolution imaging of choline-containing metabolites in living mammalian cell lines, primary hippocampal neurons and the multicellular organism C. elegans. Different subcellular distributions of choline metabolites are observed between cancer cells and non-cancer cells, which may reveal a functional difference in the choline metabolism and lipid-mediated signaling events. In neurons, choline incorporation is visualized within both soma and neurites, where choline metabolites are more evenly distributed compared to proteins. Furthermore, choline localization is also observed in the pharynx region of C. elegans larvae, consistent with its organogenesis mechanism. These applications demonstrate the potential of isotope-based stimulated Raman scattering microscopy for future choline-related disease detection and development monitoring in vivo.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1039/c3an02281aDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069604/PubMed CentralArticle
http://www.rsc.org/suppdata/an/c3/c3an02281a/c3an02281a.pdfPublisherSupporting Information
ORCID:
AuthorORCID
Hu, Fanghao0000-0002-8659-4027
Min, Wei0000-0003-2570-3557
Additional Information:© Royal Society of Chemistry 2014. Received 11th December 2013, Accepted 18th January 2014, First published on 20th January 2014. We thank M. Chalfie, Z. Chen and L. Zhang for stimulating discussion and Dr Y. Shin for providing mouse hippocampal neurons. W. Min acknowledges support from NIH Director's New Innovator Award. The authors declare no competing financial interest.
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Funding AgencyGrant Number
NIHUNSPECIFIED
Issue or Number:10
PubMed Central ID:PMC4069604
Record Number:CaltechAUTHORS:20181119-102039701
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181119-102039701
Official Citation:Hu F, Wei L, Zheng C, Shen Y, Min W. Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling. Analyst. 2014;139(10):2312-7.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:91012
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Nov 2018 19:26
Last Modified:03 Oct 2019 20:30

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