CaltechAUTHORS
  A Caltech Library Service

What can stimulated emission do for bioimaging?

Wei, Lu and Min, Wei (2013) What can stimulated emission do for bioimaging? Annals of the New York Academy of Sciences, 1293 . pp. 1-7. ISSN 0077-8923. https://resolver.caltech.edu/CaltechAUTHORS:20181119-102040019

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20181119-102040019

Abstract

Advances in bioimaging have revolutionized our ability to study life phenomena at a microscopic scale. In particular, the stimulated emission process, a universal mechanism that competes with spontaneous emission, has emerged as a powerful driving force for advancing light microscopy. The present review summarizes and compares three related techniques that each measure a different physical quantity involved in the stimulated emission process in order to tackle various challenges in light microscopy. Stimulated emission depletion microscopy, which detects the residual fluorescence after quenching, can break the diffraction‐limited resolution barrier in fluorescence microscopy. Stimulated emission microscopy is capable of imaging nonfluorescent but absorbing chromophores by detecting the intensity gain of the stimulated emission beam. Very recently, stimulated emission reduced fluorescence microscopy has been proposed, in which the reduced fluorescence due to focal stimulation is measured to extend the fundamental imaging‐depth limit of two‐photon microscopy. Thus, through ingenious spectroscopy design in distinct microscopy contexts, stimulated emission has opened up several new territories for bioimaging, allowing examination of biological structures that are ever smaller, darker, and deeper.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1111/nyas.12079DOIArticle
ORCID:
AuthorORCID
Wei, Lu0000-0001-9170-2283
Min, Wei0000-0003-2570-3557
Additional Information:© 2013 New York Academy of Sciences. The authors thank Zhixing Chen and Rafael Yuste for helpful discussions. W.M. acknowledges the start‐up funds from Columbia University, and grant support from the Kavli Institute for Brain Science. The authors declare no conflicts of interest. Issue: Blavatnik Awards for Young Scientists 2012.
Funders:
Funding AgencyGrant Number
Columbia UniversityUNSPECIFIED
Kavli Institute for Brain ScienceUNSPECIFIED
Subject Keywords:deep tissue imaging; imaging-depth limit; nonfluorescent chromophore; pump-probe microscopy; stimulated emission; superresolution
Record Number:CaltechAUTHORS:20181119-102040019
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181119-102040019
Official Citation:Wei, L. and Min, W. (2013), What can stimulated emission do for bioimaging?. Ann. N.Y. Acad. Sci., 1293: 1-7. doi:10.1111/nyas.12079
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:91015
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Nov 2018 19:10
Last Modified:23 Oct 2019 20:49

Repository Staff Only: item control page