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Pump-probe optical microscopy for imaging nonfluorescent chromophores

Wei, Lu and Min, Wei (2012) Pump-probe optical microscopy for imaging nonfluorescent chromophores. Analytical and Bioanalytical Chemistry, 403 (8). pp. 2197-2202. ISSN 1618-2642.

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Many chromophores absorb light intensely but have undetectable fluorescence. Hence microscopy techniques other than fluorescence are highly desirable for imaging these chromophores inside live cells, tissues, and organisms. The recently developed pump-probe optical microscopy techniques provide fluorescence-free contrast mechanisms by employing several fundamental light–molecule interactions including excited state absorption, stimulated emission, ground state depletion, and the photothermal effect. By using the pump pulse to excite molecules and the subsequent probe pulse to interrogate the created transient states on a laser scanning microscope, pump-probe microscopy offers imaging capability with high sensitivity and specificity toward nonfluorescent chromophores. Single-molecule sensitivity has even been demonstrated. Here we review and summarize the underlying principles of this emerging class of molecular imaging techniques.

Item Type:Article
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URLURL TypeDescription ReadCube access
Wei, Lu0000-0001-9170-2283
Min, Wei0000-0003-2570-3557
Additional Information:© Springer-Verlag 2012. Received: 5 December 2011 /Revised: 16 February 2012 / Accepted: 20 February 2012 / Published online: 13 March 2012. We acknowledge discussions with X. S. Xie, C. W. Freudiger, S. Lu, S. Chong, B. G. Saar, G. R. Holtom, M. Roeffaers, D. Fu, X. Zhang, and R. Roy. Published in the special issue Young Investigators in Analytical and Bioanalytical Science with Guest Editors S. Daunert, J. Bettmer, T. Hasegawa, Q. Wang and Y. Wei.
Subject Keywords:Pump-probe microscopy. Label-free imaging . Excited state absorption . Stimulated emission . Ground state depletion . Nonfluorescent chromophore
Issue or Number:8
Record Number:CaltechAUTHORS:20181119-102040116
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Official Citation:Wei, L. & Min, W. Anal Bioanal Chem (2012) 403: 2197.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:91016
Deposited By: George Porter
Deposited On:19 Nov 2018 19:16
Last Modified:23 Oct 2019 20:47

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