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Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag

Gatzogiannis, Evangelos and Chen, Zhixing and Wei, Lu and Wombacher, Richard and Kao, Ya-Ting and Yefremov, Grygorii and Cornish, Virginia W. and Min, Wei (2012) Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag. Chemical Communications, 48 (69). pp. 8694-8696. ISSN 1359-7345. PMCID PMC3775488. https://resolver.caltech.edu/CaltechAUTHORS:20181119-102040204

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Abstract

The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1039/c2cc33133kDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3775488/PubMed CentralArticle
http://www.rsc.org/suppdata/cc/c2/c2cc33133k/c2cc33133k.pdfPublisherSupporting Information
ORCID:
AuthorORCID
Wei, Lu0000-0001-9170-2283
Min, Wei0000-0003-2570-3557
Additional Information:© Royal Society of Chemistry 2012. Received 1st May 2012, Accepted 6th July 2012. This work was supported by the National Institutes of Health (U54 GM087519 and RC1GM091804 to V. W. C) and by the start up funds from Columbia University (to W. M.). We thank Dr Steffen Jockusch for experimental assistance. V. W. C. holds patents on the TMP-tag technology, and the technology is licensed and commercialized by Active Motif.
Funders:
Funding AgencyGrant Number
NIHU54 GM087519
NIHRC1 GM091804
Columbia UniversityUNSPECIFIED
Issue or Number:69
PubMed Central ID:PMC3775488
Record Number:CaltechAUTHORS:20181119-102040204
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181119-102040204
Official Citation:Gatzogiannis E, Chen Z, Wei L, et al. Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag. Chem Commun (Camb). 2012;48(69):8694-6.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:91017
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Nov 2018 19:06
Last Modified:23 Oct 2019 20:48

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