Schroeder, W. A. and Jones, Richard T. and Cromick, Jean and McCalla, Kathleen (1962) Chromatographic Separation of Peptides on Ion Exchange Resins. Separation of Peptides from Enzymatic Hydrolyzates of the α, β, and γ Chains of Human Hemoglobins. Analytical Chemistry, 34 (12). pp. 1570-1575. ISSN 0003-2700. doi:10.1021/ac60192a018. https://resolver.caltech.edu/CaltechAUTHORS:20181119-161053738
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Abstract
Isolation of components in a mixture is most effectively accomplished by great alteration of conditions from step to step in the separation. This principle has been applied to the separation of complex mixtures of peptides in protein hydrolyzates. Excellent results have been obtained by chromatographing first on the cation exchanger Dowex-50 and then rechromatographing on the anion exchanger Dowex-1. Volatile developers were employed throughout. Experimental procedures are presented, and results are given of their application to the separation of peptides from human hemoglobins A and F.
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| Additional Information: | © 1962 American Chemical Society. Received for review June 11, 1962. Accepted August 30, 1962. Parts of this investigation have had the assistance of J. Roger Shelton and William Fenninger. The composition and use of the pyridine-acetic acid developers on Dowex-50 were suggested by Joe R. Kimmel of the University of Utah. Presented in part at the 141st meeting of the American Chemical Society, Washington, D. C., March 1962, at the symposium honoring L. Zechmeister as recipient of the ACS Award in Chromatography and Electrophoresis. This investigation has been supported in part by a grant (H-2558) from the National Institutes of Health, United States Public Health Service. | ||||||
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| Issue or Number: | 12 | ||||||
| DOI: | 10.1021/ac60192a018 | ||||||
| Record Number: | CaltechAUTHORS:20181119-161053738 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20181119-161053738 | ||||||
| Official Citation: | Chromatographic Separation of Peptides on Ion Exchange Resins. Separation of Peptides from Enzymatic Hydrolyzates of the α, β, and γ Chains of Human Hemoglobins. W. A. Schroeder, R. T. Jones, Jean Cromick, and Kathleen McCalla Analytical Chemistry 1962 34 (12), 1570-1575 DOI: 10.1021/ac60192a018 | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 91059 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | George Porter | ||||||
| Deposited On: | 20 Nov 2018 15:48 | ||||||
| Last Modified: | 16 Nov 2021 03:38 |
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