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Diversity and structure of human T-cell receptor alpha-chain variable region genes

Klein, Michel H. and Concannon, Patrick and Everett, Margaret and Kim, Leonard D. H. and Hunkapiller, Tim and Hood, Leroy (1987) Diversity and structure of human T-cell receptor alpha-chain variable region genes. Proceedings of the National Academy of Sciences of the United States of America, 84 (19). pp. 6884-6888. ISSN 0027-8424. PMCID PMC299189.

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The nucleotide sequences of 27 T-cell receptor α-chain variable region (Vα)-containing cDNA clones isolated from a cDNA library derived from human peripheral blood lymphocytes were determined. Eighteen different Vα and 26 different joining (Jα) gene segments are utilized in these clones. The Vα gene segments belong to 12 different subfamilies, each containing from one to seven members. Comparisons with the 16 different Vα and 21 different Jα sequences previously reported suggest that the germ-line repertoires for these gene segments are greater than previously estimated. Flexibility in the sites of gene segment joining and possibly N-region diversification also contribute to human α-chain diversity. Comparisons of human Vα regions indicate a high degree of variability spread uniformly across the entire Vα region without obvious hypervariable regions. However, amino acids important for the maintenance of V gene structure are conserved.

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Hood, Leroy0000-0001-7158-3678
Additional Information:© 1987 by the National Academy of Sciences. Contributed by Leroy Hood, June 24, 1987. The authors wish to thank Dr. B. Arden for assistance in library screening, Dr. Suzanna Horvath for synthesis of oligonucleotide sequencing primers, Dr. T. Mak for providing the PY14 clone, and Cathy Elkins for assistance in the preparation of this manuscript. This work was supported in part by a grant (MT-6780) from the Medical Research Council of Canada and grants from the National Institutes of Health. M.H.K. is the recipient of a Biotechnology Retraining Award from the Medical Research Council of Canada. §These sequences are being deposited in the EMBL/GenBank data base (Bolt, Beranek, and Newman Laboratories, Cambridge, MA, and Eur. Mol. Biol. Lab., Heidelberg) (accession no. J02992). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:nucleotide sequences; variability; repertoire size
Issue or Number:19
PubMed Central ID:PMC299189
Record Number:CaltechAUTHORS:KLEpnas87
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9123
Deposited By: Tony Diaz
Deposited On:31 Oct 2007
Last Modified:02 Oct 2019 23:57

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