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Altered nucleosome spacing in newly replicated chromatin from Friend leukemia cells

Murphy, Robert F. and Wallace, R. Bruce and Bonner, James (1978) Altered nucleosome spacing in newly replicated chromatin from Friend leukemia cells. Proceedings of the National Academy of Sciences of the United States of America, 75 (12). pp. 5903-5907. ISSN 0027-8424.

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Chromatin from Friend leukemia cells labeled with [14C]thymidine for 24 hr followed by [3H]thymidine for 10 min is converted into nucleosomes by staphylococcal nuclease at only half the rate that total chromatin is converted. Polyacrylamide gel electrophoresis of nucleosomal DNA from cells labeled for 24 hr with [14C]thymidine followed by 10 min with [3H]thymidine demonstrates that the internucleosomal spacer of newly replicated chromatin is approximately 20 base pairs shorter than that of total chromatin. The implications of this difference for models of chromatin structure are discussed.

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Additional Information:© 1978 by the National Academy of Sciences. Contributed by James Bonner, September 18, 1978. phi-X174 DNA cleaved with Hae III was the gift of Richard Parker. We thank Thomas Sargent for labeling the phi-X174 DNA and for helpful discussion of this manuscript. This work was supported by U.S. Public Health Service Grant GM-13762. R.F.M. is a National Institutes of Health predoctoral trainee. R.B.W. is the holder of a Medical Research Council of Canada Centennial fellowship. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:nucleosomes; chromatin replication; staphylococcal nuclease
Issue or Number:12
Record Number:CaltechAUTHORS:MURpnas78
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9125
Deposited By: Tony Diaz
Deposited On:31 Oct 2007
Last Modified:02 Oct 2019 23:57

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