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Acetylcholine and phorbol esters inhibit potassium currents evoked by adenosine and cAMP in Xenopus oocytes

Dascal, Nathan and Lotan, Ilana and Gillo, Boaz and Lester, Henry A. and Lass, Yoram (1985) Acetylcholine and phorbol esters inhibit potassium currents evoked by adenosine and cAMP in Xenopus oocytes. Proceedings of the National Academy of Sciences of the United States of America, 82 (17). pp. 6001-6005. ISSN 0027-8424. PMCID PMC390682.

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In Xenopus laevis oocytes, adenosine and other purinergic agonists induce a K+-conductance increase that is fully mimicked by intracellular application of cAMP. Acetylcholine suppresses the K+-conductance increase caused by adenosine, by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, or by intracellular injection of cAMP. This effect of acetylcholine is not mimicked by intracellular injection of Ca2+ or of the Ca-mobilizing agent inositol 1,4,5-trisphosphate. However, adenosine and cAMP responses are inhibited by 4ß -phorbol 12,13-dibutyrate and 4ß -phorbol 12-myristate 13-acetate. These results suggest that, in Xenopus oocytes, the muscarinic inhibition of purinergic and cAMP responses is mediated through the activation of the phospholipid-dependent, Ca-activated protein kinase (protein kinase C).

Item Type:Article
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Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1985 by the National Academy of Sciences. Communicated by Howard C. Berg, April 22, 1985. We are grateful to Prof. E.M. Landau and Dr. Alison M. Gurney for the helpful discussion. This work was supported in part by grants from Recanati and Schreiber Foundations and by National Institutes of Health Grant GM29836. N.D. is a recipient of the Bantrell and Fulbright Postdoctoral Fellowships. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:muscarinic response; purinergic response; protein kinase C; second messengers; neurotransmitter interactions
Issue or Number:17
PubMed Central ID:PMC390682
Record Number:CaltechAUTHORS:DASpnas85
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9136
Deposited By: Tony Diaz
Deposited On:01 Nov 2007
Last Modified:02 Oct 2019 23:57

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