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Structure of wild-type yeast RNA polymerase II and location of Rpb4 and Rpb7

Jensen, Grant J. and Meredith, Gavin and Bushnell, David A. and Kornberg, Roger D. (1998) Structure of wild-type yeast RNA polymerase II and location of Rpb4 and Rpb7. EMBO Journal, 17 (8). pp. 2353-2358. ISSN 1460-2075. PMCID PMC1170578. doi:10.1093/emboj/17.8.2353.

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The three‐dimensional structure of wild‐type yeast RNA polymerase II has been determined at a nominal resolution of 24 Å. A difference map between this structure and that of the polymerase lacking subunits Rpb4 and Rpb7 showed these two subunits forming part of the floor of the DNA‐binding (active center) cleft, and revealed a slight inward movement of the protein domain surrounding the cleft. Surface plasmon resonance measurements showed that Rpb4 and Rpb7 stabilize a minimal pre‐initiation complex containing promoter DNA, TATA box‐binding protein (TBP), transcription factor TFIIB and the polymerase. These findings suggest that Rpb4 and Rpb7 play a role in coupling the entry of DNA into the active center cleft to closure of the cleft. Such a role can explain why these subunits are necessary for promoter‐specific transcription in vitro and for a normal stress response in vivo.

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Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 1998 EMBO. Received September 1, 1997; revised and accepted February 6, 1998. We thank Steve Orlicky and Al Edwards for providing purified recombinant Rpb4 and Rpb7 complex, and Seth Darst for helpful advice. G.J.J. was supported by a Medical Scientist Training Program grant (GM07365) provided by the National Institute of General Medical Sciences at the NIH. This research was funded by NIH grant AI21144 to R.D.K.
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Subject Keywords:electron crystallography / Rpb4 / Rpb7 / transcription
Issue or Number:8
PubMed Central ID:PMC1170578
Record Number:CaltechAUTHORS:20181213-110052088
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:91760
Deposited By: George Porter
Deposited On:13 Dec 2018 22:34
Last Modified:16 Nov 2021 03:44

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