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Analysis of somitogenesis using multiphoton laser scanning microscopy (MPLSM)

Dickinson, Mary E. and Longmuir, Kenneth J. and Fraser, Scott E. (2001) Analysis of somitogenesis using multiphoton laser scanning microscopy (MPLSM). In: Multiphoton Microscopy in the Biomedical Sciences. Proceedings of SPIE. No.4262. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, pp. 18-26. ISBN 9780819439406.

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In order to study complex cellular interactions in the developing somite and nervous system, we have been refining techniques for labeling and imaging individual cells within the living vertebrate embryo. Most recently, we have been using MPLSM to analyze cellular behaviors, such as cell migration, filopodial extension, cell process collapse, and neuron pathfinding using time-lapse microscopy in 3-dimensions (3-d). To enhance the efficiency of two-photon excitation in these samples, we have been using a Zeiss LSM 510 NLO fiber delivery system with a Grating Dispersion Compensator (GDC). This system not only offers the convenience of fiber delivery for coupling our Ti:Sapphire laser to the microscope, but also affords us precise control over the pulsewidth of the mode- locked beam. In addition, we have developed a novel peptide/non-cationic lipid gene delivery system to introduce GFP plasmid into somite cells. This approach has allowed us to generate detailed 3-d images of somite cell morphologies at various stages of somite development in a way that best preserves the vitality of the cells being imaged.

Item Type:Book Section
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Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2001 Society of Photo-Optical Instrumentation Engineers (SPIE).
Subject Keywords:Multiphoton microscopy, GFP, Somitogenesis, liposomes, filopodia, optical fibers
Series Name:Proceedings of SPIE
Issue or Number:4262
Record Number:CaltechAUTHORS:20181213-143626220
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Official Citation:Mary E. Dickinson, Kenneth J. Longmuir, Scott E. Fraser, "Analysis of somitogenesis using multiphoton laser scanning microscopy (MPLSM)," Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424570
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:91774
Deposited By: George Porter
Deposited On:19 Dec 2018 23:53
Last Modified:16 Nov 2021 03:44

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