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Metal-chelating non-canonical amino acids in metalloprotein engineering and design

Almhjell, Patrick J. and Mills, Jeremy H. (2018) Metal-chelating non-canonical amino acids in metalloprotein engineering and design. Current Opinion in Structural Biology, 51 . pp. 170-176. ISSN 0959-440X. https://resolver.caltech.edu/CaltechAUTHORS:20181220-092840906

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Abstract

The ability to rationally design metalloproteins with desired functions remains a difficult challenge despite many years of effort. Recently, the potential of using genetically encoded metal-chelating non-canonical amino acids (NCAAs) to circumvent longstanding difficulties in this field has begun to be explored. In this review, we describe the development of this approach and its application to the rational design or directed evolution of NCAA-containing metalloproteins in which the bound metal ions serve in structural roles, as catalysts, or as regulators of the assembly or disassembly of protein complexes. These successes highlight the fact that amino acids not found in nature can recapitulate the functions of their naturally occurring counterparts and suggest the promise of this nascent approach for simplifying the metalloprotein design problem.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.sbi.2018.06.001DOIArticle
ORCID:
AuthorORCID
Almhjell, Patrick J.0000-0003-0977-841X
Additional Information:© 2018 Elsevier. Available online 3 July 2018.
Record Number:CaltechAUTHORS:20181220-092840906
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181220-092840906
Official Citation:Patrick J Almhjell, Jeremy H Mills, Metal-chelating non-canonical amino acids in metalloprotein engineering and design, Current Opinion in Structural Biology, Volume 51, 2018, Pages 170-176, ISSN 0959-440X, https://doi.org/10.1016/j.sbi.2018.06.001. (http://www.sciencedirect.com/science/article/pii/S0959440X18300502)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:91929
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:20 Dec 2018 18:06
Last Modified:03 Oct 2019 20:39

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