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OH-Radical Oxidation of Lung Surfactant Protein B on Aqueous Surfaces

Enami, Shinichi and Colussi, Agustín J. (2018) OH-Radical Oxidation of Lung Surfactant Protein B on Aqueous Surfaces. Mass Spectrometry, 7 (2). pp. 1-7. ISSN 2186-5116. https://resolver.caltech.edu/CaltechAUTHORS:20181221-105357518

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Abstract

Air pollutants generate reactive oxygen species on lung surfaces. Here we report how hydroxyl radicals (·OH) injected on the surface of water react with SP-B_(1–25), a 25-residue polypeptide surrogate of human lung surfactant protein B. Our experiments consist of intersecting microjets of aqueous SP-B_(1–25) solutions with O_3/O_2/H_2O/N_2(g) gas streams that are photolyzed into ·OH(g) in situ by 266 nm laser nanosecond pulses. Surface-sensitive mass spectrometry enables us to monitor the prompt (<10 μs) and simultaneous formation of primary O_n-containing products/intermediates (n ≤ 5) triggered by the reaction of ·OH with interfacial SP-B_(1–25). We found that O-atoms from both O_3 and ·OH are incorporated into the reactive cysteine Cys_8 and Cys_(11) and tryptophan Trp_9 components of the hydrophobic N-terminus of SP-B_(1–25) that lies at the topmost layers of the air–liquid interface. Remarkably, these processes are initiated by ·OH additions rather than by H-atom abstractions from S–H, C–H, or N–H groups. By increasing the hydrophilicity of the N-terminus region of SP-B_(1–25), these transformations will impair its role as a surfactant.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.5702/massspectrometry.s0077DOIArticle
ORCID:
AuthorORCID
Enami, Shinichi0000-0002-2790-7361
Colussi, Agustín J.0000-0002-3400-4101
Additional Information:© 2018 Shinichi Enami and Agustín J. Colussi. This is an open access article distributed under the terms of Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. Received: August 21, 2018. Accepted: October 11, 2018. Published online: November 21, 2018. S. E. is grateful to the Japan Science and Technology Agency (JST) PRESTO program and the Research Foundation for Opto-Science and Technology. S. E. is indebted to Prof. Hiroshi Masuhara for his kind advices. A. J. C. acknowledges support from the National Science Foundation USA, grant AGS-1744353. SUPPORTING INFORMATION AVAILABLE: Additional data and experimental details. This material is available free of charge via the Internet.
Funders:
Funding AgencyGrant Number
Japan Science and Technology AgencyUNSPECIFIED
Research Foundation for Opto-Science and TechnologyUNSPECIFIED
NSFAGS-1744353
Subject Keywords:air pollution, lung surfactant, reactive oxygen species, interfacial reaction, protein
Issue or Number:2
Record Number:CaltechAUTHORS:20181221-105357518
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20181221-105357518
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:91959
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:21 Dec 2018 20:00
Last Modified:03 Oct 2019 20:39

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