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Methods for Collection and Characterization of Samples From Icy Environments

Bedrossian, Manuel and Deming, Jody W. and Nadeau, Jay (2018) Methods for Collection and Characterization of Samples From Icy Environments. In: Microbiology of Atypical Environments. Methods in Microbiology. No.45. Academic Press , London, pp. 293-321. ISBN 978-0-12-814604-0. http://resolver.caltech.edu/CaltechAUTHORS:20190103-131910975

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Abstract

Environments where the prevailing temperature is below 0°C include both solid (frozen) substrates, such as glacier ice and permafrost, and liquid environments such as cold springs and cryopegs. Some of these environments have low solute concentrations, whereas others have very high levels of salts and other solutes, with corresponding freezing point depression. Some have high microbial density, while others represent the limits of cell survival. All of these present specific challenges for identifying microorganisms by direct imaging or -omics techniques. In this chapter, we discuss specific cryoenvironments—sea ice, glacier ice, and permafrost—and begin with issues of sampling, from researcher safety to sample contamination and osmotic shock. We then present methods for imaging both live and fixed specimens. These include methods that may be performed in the field, such as light, fluorescence, and holographic microscopy, as well as methods that are performed upon sample return to the laboratory, such as confocal and electron microscopy. Microscope designs for field use are presented and discussed. Considerations of sample return are also presented in the context of downstream analysis.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/bs.mim.2018.05.001DOIArticle
ORCID:
AuthorORCID
Nadeau, Jay0000-0001-5258-0076
Additional Information:© 2018 Elsevier Ltd. Available online 20 June 2018.
Record Number:CaltechAUTHORS:20190103-131910975
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190103-131910975
Official Citation:Manuel Bedrossian, Jody W. Deming, Jay Nadeau, Chapter 10 - Methods for Collection and Characterization of Samples From Icy Environments, Editor(s): Volker Gurtler, Jack T. Trevors, Methods in Microbiology, Academic Press, Volume 45, 2018, Pages 293-321, ISSN 0580-9517, ISBN 9780128146040, https://doi.org/10.1016/bs.mim.2018.05.001. (http://www.sciencedirect.com/science/article/pii/S0580951718300011)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:92039
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Jan 2019 21:30
Last Modified:03 Jan 2019 21:30

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