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Fractionation of liver chromatin

Marushige, Keiji and Bonner, James (1971) Fractionation of liver chromatin. Proceedings of the National Academy of Sciences of the United States of America, 68 (12). pp. 2941-2944. ISSN 0027-8424.

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Rat liver chromatin has been fractionated into two fractions on the basis of precipitability in standard saline after mild treatment with DNase II. The major portion of liver chromatin contains little nonhistone protein and is enriched in histones, while a minor portion of such chromatin, with which RNA polymerase is associated, is highly enriched in proteins other than histone and impoverished in histones. Although the relative concentrations of the several histone species present in two chromatin fractions are identical, binding of histones to DNA in the minor fractions appears to be modified, presumably by the presence of proteins other than histones.

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Additional Information:© 1971 by the National Academy of Sciences. Contributed by James Bonner, August 27, 1971. We thank Douglas M. Fambrough for electrophoretic analyses of histones and Douglas Brutlag for sedimentation analyses of DNA, and acknowledge the assistance of Sarah Roberts Elgin. This research has been supported in part by the Herman Frasch Foundation, by USPHS Grant 13702, and in part by grants from the Rockefeller Foundation to the Laboratories for Reproductive Biology, University of North Carolina.
Subject Keywords:DNase II; differential precipitation; RNA polymerase; rat liver
Issue or Number:12
Record Number:CaltechAUTHORS:MARpnas71
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9206
Deposited By: Tony Diaz
Deposited On:27 Nov 2007
Last Modified:02 Oct 2019 23:58

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