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Sequence-specific recognition of B-DNA by oligo(N-methylpyrrolecarboxamide)s

Youngquist, R. Scott and Dervan, Peter B. (1985) Sequence-specific recognition of B-DNA by oligo(N-methylpyrrolecarboxamide)s. Proceedings of the National Academy of Sciences of the United States of America, 82 (9). pp. 2565-2569. ISSN 0027-8424. PMCID PMC397604.

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Four homologous oligopeptide-EDTA molecules, tri-, tetra, penta-, and hexa(N-methylpyrrolecarboxamide)-EDTA, in the presence of Fe(II), O2, and dithiothreitol, cleave (32)P-end-labeled restriction fragments from plasmid pBR322 DNA at common locations rich in A·T base pairs that differ in the size of the binding site. From analysis of the cleavage patterns visualized by high-resolution denaturing gel electrophoresis, the oligopeptides with three, four, five, and six N-methylpyrrolecarboxamide units, containing four, five, six, and seven amide NHs, bind sites of A·T-rich DNA consisting of five, six, seven, and eight contiguous base pairs, respectively. The general rule of n amides affording binding site sizes of n + 1 base pairs is consistent with the oligopeptides binding in the minor groove of right-handed DNA, with the amide NH groups forming bridges between the adjacent N-3 and O-2 atoms of adenine or thymine on opposite strands of the DNA helix.

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Dervan, Peter B.0000-0001-8852-7306
Additional Information:Copyright © 1985 by the National Academy of Sciences. Communicated by J. L. Beauchamp, December 10, 1984. We are grateful to the National Institutes of Health for grant support (GM-27681). This is contribution no. 7121 from the Division of Chemistry and Chemical Engineering, California Institute of Technology. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
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Caltech Division of Chemistry and Chemical Engineering7121
Issue or Number:9
PubMed Central ID:PMC397604
Record Number:CaltechAUTHORS:YOUpnas85
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:921
Deposited By: Tony Diaz
Deposited On:09 Nov 2005
Last Modified:26 Nov 2019 11:15

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