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Synthesis of a 600-nucleotide-long plus-strand DNA by virions of Moloney murine leukemia virus

Mitra, Sudha Warrier and Goff, Stephen and Gilboa, Eli and Baltimore, David (1979) Synthesis of a 600-nucleotide-long plus-strand DNA by virions of Moloney murine leukemia virus. Proceedings of the National Academy of Sciences of the United States of America, 76 (9). pp. 4355-4359. ISSN 0027-8424. doi:10.1073/pnas.76.9.4355.

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A discrete, 600-nucleotide-long plus-strand DNA has been identified among the products of reverse transcription by virions of Moloney murine leukemia virus. Its polarity was shown by hybridization to minus-strand DNA. It appears to be copied from the right end of minus-strand DNA because (i) its restriction endonuclease cleavage pattern corresponds to the redundant 600-base segment found at either end of the ultimate double-stranded reverse transcription product, (ii) its synthesis is actinomycin D sensitive, and (iii) its synthesis begins during the first hour of a reverse transcription reaction when only the right-hand end of minus-strand DNA is available as template. We therefore call this DNA plus-strong-stop DNA by analogy with the minus-strong-stop DNA copied from the left end of the viral RNA. Both strong-stop DNAs are made early during in vitro reactions and decline in concentration later, consistent with postulated roles as initiators of long minus- and plus-strand DNA. Unlike minus-strong-stop DNA, plus-strong-stop DNA remains as a double-stranded nucleic acid after its synthesis, as shown by S1 nuclease resistance. A primer to initiate plus-strong-stop DNA synthesis has not been identified; the product found thus far has no detectable RNA attached to it.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1979 by the National Academy of Sciences. Contributed by David Baltimore, June 15, 1979. This work was supported by Grant CA-14051 from the National Cancer Institute and Contract N01-CP-53562 from the Division of Cancer Cause and Prevention, National Cancer Institute, S.G. is a Postdoctoral Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. E.G. is a Chaim Weizman Postdoctoral Fellow. D.B. is an American Cancer Society Research Professor of Microbiology. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:retroviruses; reverse transcription; plus-strong-stop DNA
Issue or Number:9
Record Number:CaltechAUTHORS:MITpnas79
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9211
Deposited By: Tony Diaz
Deposited On:27 Nov 2007
Last Modified:08 Nov 2021 20:57

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