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Identification of Novel Binding Partners for Transcription Factor Emx2

Groves, Jennifer A. and Gillman, Cody and DeLay, Cierra N. and Kroll, Todd T. (2019) Identification of Novel Binding Partners for Transcription Factor Emx2. Protein Journal, 38 (1). pp. 2-11. ISSN 1572-3887.

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The mammalian homolog of Drosophila empty spiracles 2 (Emx2) is a homeobox transcription factor that plays central roles in early development of the inner ear, pelvic and shoulder girdles, cerebral cortex, and urogenital organs. The role for Emx2 is best understood within the context of the development of the neocortical region of the cortex, where Emx2 is expressed in a high posterior-medial to low anterior-lateral gradient that regulates the partitioning of the neocortex into different functional fields that perform discrete computational tasks. Despite several lines of evidence demonstrating an Emx2 concentration-dependent mechanism for establishing functional areas within the developing neocortex, little is known about how Emx2 physically carries out this role. Although several binding partners for Emx2 have been identified (including Sp8, eIF4E, and Pbx1), no screens have been used to identify potential protein binding partners for this protein. We utilized a yeast two-hybrid screen using a library constructed from embryonic mouse cDNA in an attempt to identify novel binding partners for Emx2. This initial screen isolated two potential Emx2-binding partner proteins, Cnot6l and QkI-7. These novel Emx2-binding proteins are involved in multiple levels of mRNA metabolism that including splicing, mRNA export, translation, and destruction, thus making them interesting targets for further study.

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Kroll, Todd T.0000-0002-9605-8359
Additional Information:© 2019 Springer Science+Business Media, LLC, part of Springer Nature. First Online: 09 January 2019. Mouse tissue to generate the yeast two-hybrid library was kindly provided by Dennis O’Leary at the Salk Institute for Biological Studies. Funds to conduct this work were provided by a Single Investigator Award (#19482) from the Research Corporation for Science Advancement, the Central Washington University Science Honors Program, the Central Washington University STEP program, a Central Washington University COTS Undergrad research grant, and the School of Graduate Studies and Research at Central Washington University, Ellensburg, Washington. Compliance with Ethical Standards. All authors declare that they have no conflict of interest. Research Involving Human and Animal Participants: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants by any of the authors.
Funding AgencyGrant Number
Research Corporation19482
Central Washington UniversityUNSPECIFIED
Subject Keywords:Emx2; Cnot6l; Quaking; Arealization; Development
Issue or Number:1
Record Number:CaltechAUTHORS:20190114-085628969
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Official Citation:Groves, J.A., Gillman, C., DeLay, C.N. et al. Protein J (2019) 38: 2.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:92243
Deposited By: Tony Diaz
Deposited On:14 Jan 2019 20:51
Last Modified:03 Oct 2019 20:42

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