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Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres

Mohlin, Sofie and Kunttas, Ezgi and Persson, Camilla U. and Abdel-Haq, Reem and Castillo, Aldo and Murko, Christina and Bronner, Marianne E. and Kerosuo, Laura (2019) Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres. Developmental Biology, 447 (2). pp. 137-146. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20190123-074418513

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Abstract

Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest stemness and lineage decisions as well as accompanying diseases.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.ydbio.2019.01.010DOIArticle
ORCID:
AuthorORCID
Mohlin, Sofie0000-0002-2458-3963
Abdel-Haq, Reem0000-0002-7418-5736
Murko, Christina0000-0003-2281-0031
Bronner, Marianne E.0000-0003-4274-1862
Kerosuo, Laura0000-0001-6710-3512
Additional Information:© 2019 Published by Elsevier Inc. Received 17 August 2018, Revised 9 January 2019, Accepted 9 January 2019, Available online 18 January 2019. We would like to thank Elina Fredlund for technical assistance. This work was supported by the Swedish Childhood Cancer Fund, Sweden (to SM), the Mary Bevé Foundation (to SM), Magnus Bergvall Foundation, Sweden (to SM), the Thelma Zoéga Foundation, Sweden (to SM), Hans von Foundation (to SM), the Royal Physiographic Society in Lund, Sweden (to SM), the Gyllenstiernska Krapperup Foundation, Sweden (to SM), Gunnar Nilsson Cancer Foundation, Sweden (to SM), NIH, United States, Ruth L. Kirschstein NRSA F32HD087026 (to EK), NIH R01DE024157 (to MB), the Academy of Finland, Finland (to LK), Sigrid Jusélius Foundation, Finland (to LK), and in part by the Division of Intramural Research of the National Institute of Dental and Craniofacial Research at the National Institutes of Health, Department of Health and Human Services.
Funders:
Funding AgencyGrant Number
Swedish Childhood Cancer FundUNSPECIFIED
Mary Bevé FoundationUNSPECIFIED
Magnus Bergvall FoundationUNSPECIFIED
Thelma Zoéga FoundationUNSPECIFIED
Hans von FoundationUNSPECIFIED
Royal Physiographic SocietyUNSPECIFIED
Gyllenstiernska Krapperup FoundationUNSPECIFIED
Gunnar Nilsson Cancer FoundationUNSPECIFIED
NIH Postdoctoral FellowshipF32HD087026
NIHR01DE024157
Academy of FinlandUNSPECIFIED
Sigrid Jusélius FoundationUNSPECIFIED
Subject Keywords:Crestospheres; Neural crest stem cells; Trunk neural crest; Stem cell maintenance; Multipotency, self-renewal
Record Number:CaltechAUTHORS:20190123-074418513
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190123-074418513
Official Citation:Sofie Mohlin, Ezgi Kunttas, Camilla U. Persson, Reem Abdel-Haq, Aldo Castillo, Christina Murko, Marianne E. Bronner, Laura Kerosuo, Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres, Developmental Biology, Volume 447, Issue 2, 2019, Pages 137-146, ISSN 0012-1606, https://doi.org/10.1016/j.ydbio.2019.01.010. (http://www.sciencedirect.com/science/article/pii/S001216061830558X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:92408
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Jan 2019 17:32
Last Modified:25 Mar 2019 22:25

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