CaltechAUTHORS
  A Caltech Library Service

The SUMO Ligase Su(var)2-10 Controls Hetero- and Euchromatic Gene Expression via Establishing H3K9 Trimethylation and Negative Feedback Regulation

Ninova, Maria and Godneeva, Baira and Chen, Yung-Chia Ariel and Luo, Yicheng and Prakash, Sharan J. and Jankovics, Ferenc and Erdélyi, Miklós and Aravin, Alexei A. and Fejes Tóth, Katalin (2020) The SUMO Ligase Su(var)2-10 Controls Hetero- and Euchromatic Gene Expression via Establishing H3K9 Trimethylation and Negative Feedback Regulation. Molecular Cell, 77 (3). pp. 571-585. ISSN 1097-2765. PMCID PMC7007874. https://resolver.caltech.edu/CaltechAUTHORS:20190130-121936593

[img] PDF - Submitted Version
Creative Commons Attribution Non-commercial No Derivatives.

20Mb
[img] PDF (Figures S1–S5) - Supplemental Material
See Usage Policy.

9Mb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20190130-121936593

Abstract

Сhromatin is critical for genome compaction and gene expression. On a coarse scale, the genome is divided into euchromatin, which harbors the majority of genes and is enriched in active chromatin marks, and heterochromatin, which is gene-poor but repeat-rich. The conserved molecular hallmark of heterochromatin is the H3K9me3 modification, which is associated with gene silencing. We found that in Drosophila, deposition of most of the H3K9me3 mark depends on SUMO and the SUMO ligase Su(var)2-10, which recruits the histone methyltransferase complex SetDB1/Wde. In addition to repressing repeats, H3K9me3 influences expression of both hetero- and euchromatic host genes. High H3K9me3 levels in heterochromatin are required to suppress spurious transcription and ensure proper gene expression. In euchromatin, a set of conserved genes is repressed by Su(var)2-10/SetDB1-induced H3K9 trimethylation, ensuring tissue-specific gene expression. Several components of heterochromatin are themselves repressed by this pathway, providing a negative feedback mechanism to ensure chromatin homeostasis.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.molcel.2019.09.033DOIArticle
https://doi.org/10.1101/533232DOIDiscussion Paper
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007874/PubMed CentralArticle
ORCID:
AuthorORCID
Ninova, Maria0000-0001-5051-5502
Luo, Yicheng0000-0003-3704-2389
Jankovics, Ferenc0000-0001-9697-4472
Erdélyi, Miklós0000-0002-9501-5752
Fejes Tóth, Katalin0000-0001-6558-2636
Alternate Title:SUMO ligase Su(var)2-10 controls eu- and heterochromatic gene expression via establishment of H3K9 trimethylation and negative feedback regulation
Additional Information:© 2019 Elsevier Inc. Received 13 February 2019, Revised 11 June 2019, Accepted 26 September 2019, Available online 31 December 2019. We thank members of the Fejes Tóth and Aravin labs for discussion. We are grateful to the Bloomington Stock Center and Julius Brennecke for providing flies. We thank Igor Antoshechkin (California Institute of Technology) for help with sequencing. This work was supported by grants from the National Research, Development and Innovation Office (NKFI-K117010, GINOP-2.3.2-15-2016-00032, and GINOP-2.3.2.-15-2016-00001) to (M.E. and F.J.), the NIH (R01 GM097363), the Ministry of Education and Science of the Russian Federation (14.W03.31.0007), and the Packard Fellowship Awards (to A.A.A.), and the NIH (R01GM110217) and Ellison Medical Foundation Awards (to K.F.T.). Author Contributions: M.N., A.A.A., and K.F.T. designed the experiments and wrote the manuscript. M.N., B.G., Y.L., Y.-C.A.C., and S.J.P. executed the experiments. F.J. and M.E. generated reagents. M.N. performed the computational analysis and interpretation of the data. The authors declare no competing interests.
Funders:
Funding AgencyGrant Number
National Research, Development and Innovation Office Hungary (NKFI)NKFI-K117010
National Research, Development and Innovation Office Hungary (NKFI)GINOP-2.3.2-15-2016-00032
National Research, Development and Innovation Office Hungary (NKFI)GINOP-2.3.2.-15-2016-00001
NIHR01 GM097363
Ministry of Education and Science of the Russian Federation14.W03.31.0007
David and Lucile Packard FoundationUNSPECIFIED
NIHR01GM110217
Ellison Medical FoundationUNSPECIFIED
Subject Keywords:chromatin; heterochromatin; epigenetics; gene regulation; transcriptional repression; transposons; germline; cell fate maintenance; SUMO; H3K9me3
Issue or Number:3
PubMed Central ID:PMC7007874
Record Number:CaltechAUTHORS:20190130-121936593
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190130-121936593
Official Citation:Maria Ninova, Baira Godneeva, Yung-Chia Ariel Chen, Yicheng Luo, Sharan J. Prakash, Ferenc Jankovics, Miklós Erdélyi, Alexei A. Aravin, Katalin Fejes Tóth, The SUMO Ligase Su(var)2-10 Controls Hetero- and Euchromatic Gene Expression via Establishing H3K9 Trimethylation and Negative Feedback Regulation, Molecular Cell, Volume 77, Issue 3, 2020, Pages 571-585.e4, ISSN 1097-2765, https://doi.org/10.1016/j.molcel.2019.09.033. (http://www.sciencedirect.com/science/article/pii/S1097276519307592)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:92523
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:31 Jan 2019 00:14
Last Modified:11 Feb 2020 23:47

Repository Staff Only: item control page