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Geochemical and metagenomic characterization of Jinata Onsen, a Proterozoic-analog hot spring, reveals novel microbial diversity including iron-tolerant phototrophs and thermophilic lithotrophs

Ward, Lewis M. and Idei, Airi and Nakagawa, Mayuko and Ueno, Yuichiro and Fischer, Woodward W. and McGlynn, Shawn E. (2018) Geochemical and metagenomic characterization of Jinata Onsen, a Proterozoic-analog hot spring, reveals novel microbial diversity including iron-tolerant phototrophs and thermophilic lithotrophs. . (Unpublished) http://resolver.caltech.edu/CaltechAUTHORS:20190130-121936697

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Abstract

Hydrothermal systems, including terrestrial hot springs, contain diverse geochemical conditions that vary over short spatial scales due to progressive interaction between the reducing hydrothermal fluids, the oxygenated atmosphere, and in some cases seawater. At Jinata Onsen, on Shikinejima Island, Japan, an intertidal, anoxic, iron-rich hot spring mixes with the oxygenated atmosphere and seawater over short spatial scales, creating a diversity of chemical potentials and redox pairs over a distance ~10 m. We characterized the geochemical conditions along the outflow of Jinata Onsen as well as the microbial communities present in biofilms, mats, and mineral crusts along its traverse via 16S rDNA amplicon and genome-resolved shotgun metagenomic sequencing. The microbial community changed significantly downstream as temperatures and dissolved iron concentrations decreased and dissolved oxygen increased. Near the spring source, biomass is limited relative to downstream, and primary productivity may be fueled by oxidation of ferrous iron and molecular hydrogen by members of the Zetaproteobacteria and Aquificae. Downstream, the microbial community is dominated by oxygenic Cyanobacteria. Cyanobacteria are abundant and active even at ferrous iron concentrations of ~150 µM, which challenges the idea that iron toxicity limited cyanobacterial expansion in Precambrian oceans. Several novel lineages of Bacteria are also present at Jinata Onsen, including previously uncharacterized members of the Chloroflexi and Caldithrichaeota phyla, positioning Jinata Onsen as a valuable site for future characterization of these clades.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/428698DOIDiscussion Paper
https://www.biorxiv.org/content/10.1101/428698v2.supplementary-materialPublisherSupporting Information
ORCID:
AuthorORCID
Ward, Lewis M.0000-0002-9290-2567
Fischer, Woodward W.0000-0002-8836-3054
McGlynn, Shawn E.0000-0002-8199-7011
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. Data availability: Raw 16S rDNA, raw metagenomic sequence data, and MAGs have been uploaded and made publicly available on NCBI under Project Number PRJNA392119 (genome accession numbers can be found in Supplemental Table 6).
Record Number:CaltechAUTHORS:20190130-121936697
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190130-121936697
Official Citation:Geochemical and metagenomic characterization of Jinata Onsen, a Proterozoic-analog hot spring, reveals novel microbial diversity including iron-tolerant phototrophs and thermophilic lithotrophs Lewis M Ward, Airi Idei, Mayuko Nakagawa, Yuichiro Ueno, Woodward W. Fischer, Shawn E McGlynn bioRxiv 428698; doi: https://doi.org/10.1101/428698
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:92524
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:31 Jan 2019 00:15
Last Modified:31 Jan 2019 00:15

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