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Phototrophic Methane Oxidation in a Member of the Chloroflexi Phylum

Ward, Lewis M. and Shih, Patrick M. and Hemp, James and Kakegawa, Takeshi and Fischer, Woodward W. and McGlynn, Shawn E. (2019) Phototrophic Methane Oxidation in a Member of the Chloroflexi Phylum. . (Unpublished) http://resolver.caltech.edu/CaltechAUTHORS:20190130-121936798

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Abstract

Biological methane cycling plays an important role in Earth's climate and the global carbon cycle, with biological methane oxidation (methanotrophy) modulating methane release from numerous environments including soils, sediments, and water columns. Methanotrophy is typically coupled to aerobic respiration or anaerobically via the reduction of sulfate, nitrate, or metal oxides, and while the possibility of coupling methane oxidation to phototrophy (photomethanotrophy) has been proposed, no organism has ever been described that is capable of this metabolism. Here we described a new bacterial genome from a member of the Chloroflexi phylum--termed here Candidatus Chlorolinea photomethanotrophicum--with cooccurring methanotrophy and phototrophy pathways, suggesting a novel link between these two metabolisms. Recovered as a metagenome-assembled genome from microbial mats in an iron-rich hot spring in Japan, Ca. "C. photomethanotrophicum" forms a new lineage within the Chloroflexi phylum and expands the known metabolic diversity of this already diverse clade. Ca. "C. photomethanotrophicum" appears to be metabolically versatile, capable of phototrophy (via a Type 2 reaction center), aerobic respiration, nitrite reduction, oxidation of methane and carbon monoxide, and potentially carbon fixation via a novel pathway composed of hybridized components of the serine cycle and the 3-hydroxypropionate bicycle. The biochemical network of this organism is constructed from components from multiple organisms and pathways, further demonstrating the modular nature of metabolic machinery and the ecological and evolutionary importance of horizontal gene transfer in the establishment of novel pathways.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/531582DOIDiscussion Paper
https://www.biorxiv.org/content/10.1101/531582v1.supplementary-materialPublisherSupporting Information
ORCID:
AuthorORCID
Ward, Lewis M.0000-0002-9290-2567
Shih, Patrick M.0000-0002-2119-3345
Fischer, Woodward W.0000-0002-8836-3054
McGlynn, Shawn E.0000-0002-8199-7011
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. LMW acknowledges support from NASA NESSF (#NNX16AP39H), NSF (#OISE 1639454), NSF GROW (#DGE 1144469), the Earth-Life Science Institute Origins Network (EON), and the Agouron Institute. P.M.S. was supported by The Branco Weiss Fellowship - Society in Science from ETH Zurich. WWF acknowledges the generous support of the Caltech Center for Environment Microbe Interactions, NASA Exobiology (#NNX16AJ57G), and the Simons Foundation Collaboration on the Origins of Life (SCOL). SEM is supported by NSF Award 1724300, JSPS KAKENHI Grant Number 18H01325, and the Research Foundation for Opto-Science and Technology.
Group:Caltech Center for Environmental Microbial Interactions (CEMI)
Funders:
Funding AgencyGrant Number
NASA Earth and Space Science FellowshipNNX16AP39H
NSFOISE-1639454
NSF Graduate Research FellowshipDGE-1144469
Earth-Life Science Institute Origins Network (EON)UNSPECIFIED
Agouron InstituteUNSPECIFIED
ETH ZurichUNSPECIFIED
Caltech Center for Environmental Microbial Interactions (CEMI)UNSPECIFIED
NASANNX16AJ57G
Simons FoundationUNSPECIFIED
NSFEF-1724300
Japan Society for the Promotion of Science (JSPS)18H01325
Research Foundation for Opto-Science and TechnologyUNSPECIFIED
Subject Keywords:lateral gene transfer, metagenomics, photosynthesis, greenhouse gas
Record Number:CaltechAUTHORS:20190130-121936798
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190130-121936798
Official Citation:Phototrophic Methane Oxidation in a Member of the Chloroflexi Phylum Lewis Ward, Patrick M Shih, James Hemp, Takeshi Kakegawa, Woodward W Fischer, Shawn E McGlynn bioRxiv 531582; doi: https://doi.org/10.1101/531582
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:92525
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:31 Jan 2019 00:16
Last Modified:31 Jan 2019 00:16

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