CaltechAUTHORS
  A Caltech Library Service

A novel mesocosm set-up reveals strong methane emission reduction in submerged peat moss Sphagnum cuspidatum by tightly associated methanotrophs

Kox, Martine A. R. and Smolders, Alfons J. P. and Speth, Daan R. and Lamers, Leon P. M. and Op den Camp, Huub J. M. and Jetten, Mike S. M. and Van Kessel, Maartje A. H. J. (2019) A novel mesocosm set-up reveals strong methane emission reduction in submerged peat moss Sphagnum cuspidatum by tightly associated methanotrophs. . (Unpublished) http://resolver.caltech.edu/CaltechAUTHORS:20190201-092223925

[img] PDF - Submitted Version
Creative Commons Attribution Non-commercial No Derivatives.

1003Kb
[img] PDF (Supplementary tables and figures) - Supplemental Material
Creative Commons Attribution Non-commercial No Derivatives.

1262Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20190201-092223925

Abstract

Wetlands present the largest natural sources of methane (CH_4) and their potential CH_4 emissions greatly vary due to the activity of CH_4-oxidizing bacteria associated with wetland plant species. In this study, the association of CH_4-oxidizing bacteria with submerged Sphagnum peat mosses was studied, followed by the development of a novel mesocosm set-up. This set-up enabled the precise control of CH_4 input and allowed for monitoring the dissolved CH_4in a Sphagnum moss layer while mimicking natural conditions. Two mesocosm set-ups were used in parallel: one containing a Sphagnum moss layer in peat water, and a control only containing peat water. Moss-associated CH_4 oxidizers in the field could reduce net CH_4 emission up to 93%, and in the mesocosm set-up up to 31%. Furthermore, CH_4 oxidation was only associated with Sphagnum, and did not occur in peat water. Especially methanotrophs containing a soluble methane monooxygenase enzyme were significantly enriched during the 32 day mesocosm incubations. Together these findings showed the new mesocosm setup is very suited to study CH_4 cycling in submerged Sphagnum moss community under controlled conditions. Furthermore, the tight associated between Sphagnum peat mosses and methanotrophs can significantly reduce CH_4 emissions in submerged peatlands.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/536268DOIDiscussion Paper
https://www.biorxiv.org/content/10.1101/536268v1.supplementary-materialPublisherSupporting Information
ORCID:
AuthorORCID
Speth, Daan R.0000-0002-2361-5935
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. We thank Nardy Kip for support with the initial design of the mesocosms. Tijs van den Bosch is thanked for helping out with the 16S rRNA sequencing. The General Instruments department at the Faculty of Science at Radboud University, especially Paul van der Ven and Sebastian Krosse are thanked for measuring elemental composition of the water samples and determining stable isotope contents. MARK was supported by European Research Council Advanced Grant Ecomom 339880 to MSMJ, who was further supported by the Netherlands Organization for Scientific Research (SIAM Gravitation grant 024 002 002 and Spinoza Award). MAHJvK was supported by NWO Veni grant (016.veni.192.062). HJMOdC was supported by European Research Council Advanced Grant VOLCANO 669371. Availability of data and materials: All sequencing data has been deposited in the NCBI SRA database, project number PRJNA517391. The authors declare no competing financial interests.
Funders:
Funding AgencyGrant Number
European Research Council (ERC)339880
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)024 002 002
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)016.veni.192.062
European Research Council (ERC)669371
Subject Keywords:Methanotrophy, Peatland, Sphagnum moss, Methane cycle, mesocosm, climate change, soluble methane monooxygenase
Record Number:CaltechAUTHORS:20190201-092223925
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190201-092223925
Official Citation:A novel mesocosm set-up reveals strong methane emission reduction in submerged peat moss Sphagnum cuspidatum by tightly associated methanotrophs. Martine A.R. Kox, Alfons J.P. Smolders, Daan R. Speth, Leon P.M. Lamers, Huub J.M. Op den Camp, Mike S.M. Jetten, Maartje A.H.J. Van Kessel bioRxiv 536268; doi: https://doi.org/10.1101/536268
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:92556
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:01 Feb 2019 18:06
Last Modified:01 Feb 2019 18:06

Repository Staff Only: item control page