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Development of Next-Generation Fluorescent Turn-On Sensor to Simultaneously Detect and Detoxify Mercury in Living Samples

Malek, Abdul and Bera, Kallol and Biswas, Shrutidhara and Perumal, Govindaraj and Das, Anand Kant and Doble, Mukesh and Thomas, Tiju and Prasad, Edamana (2019) Development of Next-Generation Fluorescent Turn-On Sensor to Simultaneously Detect and Detoxify Mercury in Living Samples. Analytical Chemistry, 91 (5). pp. 3533-3538. ISSN 0003-2700. http://resolver.caltech.edu/CaltechAUTHORS:20190204-095837869

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Abstract

Strategies for simultaneous detection and detoxification of Hg^(2+) using a single sensor from biological and environmental samples are limited and have not been realized in living organisms so far. We report a highly selective, small molecule “turn-on” fluorescent sensor, PYDMSA, based on the cationic dye Pyronin Y (PY) and chelating agent meso-2,3-dimercaptosuccinic acid (DMSA) for the simultaneous detection and detoxification of inorganic mercury (Hg^(2+)). After Hg^(2+) detection, concomitant detoxification was carried out with sufficient efficacy in living samples, which makes the sensor unique. PYDMSA exhibits high selectivity for Hg^(2+) over other competing metal ions with an experimental detection limit of ∼300 pM in aqueous buffer solution. When PYDMSA reacts with Hg2+, the CS–C^9 bond in the sensor gets cleaved. This results in the “turn-on” response of the fluorescence probe with a concomitant release of one equivalent of water-soluble Hg^(2+)–DMSA complex which leads to a synchronous detoxifying effect. The sensor by itself is nontoxic to cells in culture and has been used to monitor the real-time uptake of Hg^(2+) in live cells and zebrafish larvae. Thus, PYDMSA is a unique sensor which can be used to detect and detoxify mercury at the same time in living samples.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acs.analchem.8b05268DOIArticle
https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.8b05268PublisherSupporting Information
ORCID:
AuthorORCID
Malek, Abdul0000-0002-1608-9375
Thomas, Tiju0000-0001-8322-6387
Prasad, Edamana0000-0002-6643-1407
Additional Information:© 2019 American Chemical Society. Received: November 14, 2018; Accepted: February 4, 2019; Published: February 4, 2019. We thank the Department of Science and Technology (Government of India) for financial support (DST FILE NO. YSS/2015/001712 and DST 11-IFA-PH-07). A.M. thanks IIT Madras for an HTRA fellowship. We sincerely thank Dr. S. Maiti (TIFR) for allowing us to use his cell culture facility and Dr. M. Sonawane (TIFR) for allowing us to use his fish culture facility. Author Contributions: A.M. and K.B. contributed equally. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
Department of Science and Technology (India)YSS/2015/001712
Department of Science and Technology (India)11-IFA-PH-07
Indian Institute Of TechnologyUNSPECIFIED
Record Number:CaltechAUTHORS:20190204-095837869
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190204-095837869
Official Citation:Development of a Next-Generation Fluorescent Turn-On Sensor to Simultaneously Detect and Detoxify Mercury in Living Samples. Abdul Malek, Kallol Bera, Shrutidhara Biswas, Govindaraj Perumal, Anand Kant Das, Mukesh Doble, Tiju Thomas, and Edamana Prasad. Analytical Chemistry 2019 91 (5), 3533-3538. DOI: 10.1021/acs.analchem.8b05268
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:92621
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Feb 2019 18:08
Last Modified:05 Mar 2019 23:46

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