Malek, Abdul and Bera, Kallol and Biswas, Shrutidhara and Perumal, Govindaraj and Das, Anand Kant and Doble, Mukesh and Thomas, Tiju and Prasad, Edamana (2019) Development of Next-Generation Fluorescent Turn-On Sensor to Simultaneously Detect and Detoxify Mercury in Living Samples. Analytical Chemistry, 91 (5). pp. 3533-3538. ISSN 0003-2700. doi:10.1021/acs.analchem.8b05268. https://resolver.caltech.edu/CaltechAUTHORS:20190204-095837869
![]() |
PDF
- Accepted Version
See Usage Policy. 1MB |
![]() |
PDF (Characterization of PYDMSA by FTIR spectra; 1H and 13C NMR of PYDMSA; HRMS of PYDMSA; UV–visible spectroscopic characterization of PYDMSA; color change of the solution containing PYDMSA in the presence of Hg2+; time-dependent response of PYDMSA to Hg2+...)
- Supplemental Material
See Usage Policy. 1MB |
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20190204-095837869
Abstract
Strategies for simultaneous detection and detoxification of Hg^(2+) using a single sensor from biological and environmental samples are limited and have not been realized in living organisms so far. We report a highly selective, small molecule “turn-on” fluorescent sensor, PYDMSA, based on the cationic dye Pyronin Y (PY) and chelating agent meso-2,3-dimercaptosuccinic acid (DMSA) for the simultaneous detection and detoxification of inorganic mercury (Hg^(2+)). After Hg^(2+) detection, concomitant detoxification was carried out with sufficient efficacy in living samples, which makes the sensor unique. PYDMSA exhibits high selectivity for Hg^(2+) over other competing metal ions with an experimental detection limit of ∼300 pM in aqueous buffer solution. When PYDMSA reacts with Hg2+, the CS–C^9 bond in the sensor gets cleaved. This results in the “turn-on” response of the fluorescence probe with a concomitant release of one equivalent of water-soluble Hg^(2+)–DMSA complex which leads to a synchronous detoxifying effect. The sensor by itself is nontoxic to cells in culture and has been used to monitor the real-time uptake of Hg^(2+) in live cells and zebrafish larvae. Thus, PYDMSA is a unique sensor which can be used to detect and detoxify mercury at the same time in living samples.
Item Type: | Article | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| |||||||||
ORCID: |
| |||||||||
Additional Information: | © 2019 American Chemical Society. Received: November 14, 2018; Accepted: February 4, 2019; Published: February 4, 2019. We thank the Department of Science and Technology (Government of India) for financial support (DST FILE NO. YSS/2015/001712 and DST 11-IFA-PH-07). A.M. thanks IIT Madras for an HTRA fellowship. We sincerely thank Dr. S. Maiti (TIFR) for allowing us to use his cell culture facility and Dr. M. Sonawane (TIFR) for allowing us to use his fish culture facility. Author Contributions: A.M. and K.B. contributed equally. The authors declare no competing financial interest. | |||||||||
Funders: |
| |||||||||
Issue or Number: | 5 | |||||||||
DOI: | 10.1021/acs.analchem.8b05268 | |||||||||
Record Number: | CaltechAUTHORS:20190204-095837869 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20190204-095837869 | |||||||||
Official Citation: | Development of a Next-Generation Fluorescent Turn-On Sensor to Simultaneously Detect and Detoxify Mercury in Living Samples. Abdul Malek, Kallol Bera, Shrutidhara Biswas, Govindaraj Perumal, Anand Kant Das, Mukesh Doble, Tiju Thomas, and Edamana Prasad. Analytical Chemistry 2019 91 (5), 3533-3538. DOI: 10.1021/acs.analchem.8b05268 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 92621 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Tony Diaz | |||||||||
Deposited On: | 04 Feb 2019 18:08 | |||||||||
Last Modified: | 16 Nov 2021 03:52 |
Repository Staff Only: item control page