CaltechAUTHORS
  A Caltech Library Service

Optimal Experiment Design and Leveraging Competition for Shared Resources in Cell-Free Extracts

Halter, Wolfgang and Allgöwer, Frank and Murray, Richard M. and Gyorgy, Andras (2018) Optimal Experiment Design and Leveraging Competition for Shared Resources in Cell-Free Extracts. In: 2018 IEEE Conference on Decision and Control (CDC). IEEE , Piscataway, NJ, pp. 1872-1879. ISBN 978-1-5386-1395-5. http://resolver.caltech.edu/CaltechAUTHORS:20190204-130950357

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20190204-130950357

Abstract

The fact that genes compete for shared cellular resources poses a fundamental challenge when identifying parameters of genetic parts. A recently developed model of gene expression tackles this problem by explicitly accounting for resource competition. In addition to accurately describing experimental data, this model only depends on a small number of easily identifiable parameters with clear physical interpretation. Based on this model, we outline a procedure to select the optimal set of experiments to characterize biomolecular parts in synthetic biology. Additionally, we reveal the role competition for shared resources plays, provide guidelines how to minimize its detrimental effects, and how to leverage this phenomenon to extract the most information about unknown parameters. To illustrate the results, we consider the case of part characterization in cell-free extracts, treat plasmid DNA concentrations as decision variables, and demonstrate the significant performance difference between naive and optimal experiment design.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1109/CDC.2018.8619039DOIArticle
ORCID:
AuthorORCID
Murray, Richard M.0000-0002-5785-7481
Additional Information:© 2018 IEEE. The authors are grateful for Vipul Singhal and William Poole for the insightful comments and thought provoking discussions.
Record Number:CaltechAUTHORS:20190204-130950357
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190204-130950357
Official Citation:W. Halter, F. Allgower, R. M. Murray and A. Gyorgy, "Optimal Experiment Design and Leveraging Competition for Shared Resources in Cell-Free Extracts," 2018 IEEE Conference on Decision and Control (CDC), FL, USA, 2018, pp. 1872-1879. doi: 10.1109/CDC.2018.8619039
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:92633
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Feb 2019 21:15
Last Modified:04 Feb 2019 21:15

Repository Staff Only: item control page