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B lymphocyte-specific protein binding near an immunoglobulin kappa-chain gene J segment

Weaver, David and Baltimore, David (1987) B lymphocyte-specific protein binding near an immunoglobulin kappa-chain gene J segment. Proceedings of the National Academy of Sciences of the United States of America, 84 (6). pp. 1516-1520. ISSN 0027-8424. doi:10.1073/pnas.84.6.1516.

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Nuclear extracts from pre-B and B cell lines contain a nuclear DNA binding protein (kappa locus protein, KLP) that specifically recognizes a DNA sequence in the immunoglobulin kappa light chain joining (J) segment gene region. KLP is not observed in mature B cells, T cells, or nonlymphoid cell types. Two tandem binding sites for KLP designated KI and KII have been identified by methylation interference analysis to be immediately proximal to the J-kappa-1 nonamer-heptamer recognition sequences and separated by 38 base pairs from each other. Fragments of DNA containing KI and KII sites compete for binding to KLP, and both protein-DNA complexes have the same electrophoretic mobility. Other flanking sequences of immunoglobulin gene fragments do not bind to KLP. The position of KLP-DNA binding and its tissue-specific expression suggest that it may be involved in the regulation of lymphoid gene DNA rearrangements by targeting recombinase to the kappa-chain gene region.

Item Type:Article
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URLURL TypeDescription CentralArticle
Baltimore, David0000-0001-8723-8190
Additional Information:© 1987 by the National Academy of Sciences. Contributed by David Baltimore, November 17, 1986. We thank Lou Staudt, Ranjan Sen, Nancy Speck, and Mike Gilman for providing some of the nuclear extracts tested. This work was supported by a grant from the American Cancer Society to D.B. D.W. was supported by the Helen Hay Whitney Foundation. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
American Cancer SocietyUNSPECIFIED
Helen Hay Whitney FoundationUNSPECIFIED
Subject Keywords:DNA binding protein; B cell-specific protein; DNA rearrangement
Issue or Number:6
Record Number:CaltechAUTHORS:WEApnas87
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9273
Deposited By: Tony Diaz
Deposited On:05 Dec 2007
Last Modified:08 Nov 2021 20:57

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