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Symmetrical In Vivo transcription of mitochondrial DNA in HeLa cells

Aloni, Y. and Attardi, G. (1971) Symmetrical In Vivo transcription of mitochondrial DNA in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America, 68 (8). pp. 1757-1761. ISSN 0027-8424.

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RNA·DNA hybridization experiments utilizing separated strands of HeLa mitochondrial DNA and mit-RNA from HeLa cells exposed to short pulses of [5-3H]uridine have shown that the labeled RNA hybridizes with both the light (L) and the heavy (H) strand, though to a different relative extent depending upon the labeling time. Thus, hybridization of pulse-labeled RNA is about equal with the two strands when the pulse is very short (1-5 min), and becomes more and more predominant with the H strand with increasing pulse length. Pulse-labeled fast-sedimenting mit-RNA forms RNase-resistant double-stranded structures up to more than 5 µm long when self-annealed or annealed with an excess of unlabeled mit-RNA. These observations and the previous evidence of complete transcription of the H strand strongly suggest that mit-DNA is transcribed in HeLa cells symmetrically over a considerable portion of its length, with the transcript of the L strand being rapidly degraded or otherwise removed from the mitochondrial fraction.

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Additional Information:Copyright © 1971 by the National Academy of Sciences. Communicated by Renato Dulbecco, May 25, 1971. These investigations were supported by a research grant from the U.S. Public Health Service (GM-11726) and by a Dernham Fellowship of the American Cancer Society to one of us (Y. A.). The help of Mrs. L. Wenzel and Mrs. B. Keeley is gratefully acknowledged. This is article VII in the series Expression of the Mitochondrial Genome in HeLa Cells. Paper VI is ref. 10.
Subject Keywords:electron microscopy
Issue or Number:8
Record Number:CaltechAUTHORS:ALOpnas71
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:928
Deposited By: Tony Diaz
Deposited On:09 Nov 2005
Last Modified:02 Oct 2019 22:38

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