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Adhesive L1CAM-Robo Signaling Aligns Growth Cone F-Actin Dynamics to Promote Axon-Dendrite Fasciculation in C. elegans

Chen, Chun-Hao and Hsu, Hao-Wei and Chang, Yun-Hsuan and Pan, Chun-Liang (2019) Adhesive L1CAM-Robo Signaling Aligns Growth Cone F-Actin Dynamics to Promote Axon-Dendrite Fasciculation in C. elegans. Developmental Cell, 48 (2). pp. 215-228. ISSN 1534-5807. https://resolver.caltech.edu/CaltechAUTHORS:20190213-152836749

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Abstract

Neurite fasciculation through contact-dependent signaling is important for the wiring and function of the neuronal circuits. Here, we describe a type of axon-dendrite fasciculation in C. elegans, where proximal dendrites of the nociceptor PVD adhere to the axon of the ALA interneuron. This axon-dendrite fasciculation is mediated by a previously uncharacterized adhesive signaling by the ALA membrane signal SAX-7/L1CAM and the PVD receptor SAX-3/Robo but independent of Slit. L1CAM physically interacts with Robo and instructs dendrite adhesion in a Robo-dependent manner. Fasciculation mediated by L1CAM-Robo signaling aligns F-actin dynamics in the dendrite growth cone and facilitates dynamic growth cone behaviors for efficient dendrite guidance. Disruption of PVD dendrite fasciculation impairs nociceptive mechanosensation and rhythmicity in body curvature, suggesting that dendrite fasciculation governs the functions of mechanosensory circuits. Our work elucidates the molecular mechanisms by which adhesive axon-dendrite signaling shapes the construction and function of sensory neuronal circuits.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.devcel.2018.10.028DOIArticle
ORCID:
AuthorORCID
Pan, Chun-Liang0000-0003-0108-3138
Additional Information:© 2018 Elsevier Inc. Received 22 May 2018, Revised 18 September 2018, Accepted 26 October 2018, Available online 13 December 2018. We thank Nelson Ramirez and Hannes Bülow for sharing data and suggestions before publication and Paul Sternberg for the slt-1(eh15) strain. The authors are grateful to Hua-Man Hsu for her assistance in confocal microscopy and the First and Third Core Labs, National Taiwan University College of Medicine, for imaging, flow cytometry, and cell sorting. We thank Chih-Chiang Chan for providing the pAc5.1 vector. Some of the strains used in this study are provided by the Caenorhabditis Genetics Center, which is supported by NIH Office of Research Infrastructure Programs (P40OD010440), and by the National BioResources Project (NBRP), Japan. This study was funded by the Ministry of Science and Technology (MOST), Taiwan, to C.-L.P. (MOST 106-2320-B-002-051-MY3) and a postdoctoral fellowship to C.-H.C. (MOST 106-2811-B-002-134). Author Contributions: Conceptualization, C.-H.C. and C.-L.P.; Investigation, C.-H.C., H.-W.H., Y.-H.C., and C.-L.P.; Writing – Original Draft, C.-H.C. and C.-L.P.; Writing – Review & Editing, C.-H.C. and C.-L.P.; Funding Acquisition, C.-L.P.; Supervision, C.-L.P.
Funders:
Funding AgencyGrant Number
NIHP40OD010440
National BioResources ProjectUNSPECIFIED
Ministry of Science and Technology (Taipei)MOST 106-2320-B-002-051-MY3
Ministry of Science and Technology (Taipei)MOST 106-2811-B-002-134
Subject Keywords:dendrite; axon; fasciculation; somatosensation; C. elegans; Robo; cell adhesion molecule; F-actin; cytoskeleton; growth cone
Issue or Number:2
Record Number:CaltechAUTHORS:20190213-152836749
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190213-152836749
Official Citation:Chun-Hao Chen, Hao-Wei Hsu, Yun-Hsuan Chang, Chun-Liang Pan, Adhesive L1CAM-Robo Signaling Aligns Growth Cone F-Actin Dynamics to Promote Axon-Dendrite Fasciculation in C. elegans, Developmental Cell, Volume 48, Issue 2, 2019, Pages 215-228.e5, ISSN 1534-5807, https://doi.org/10.1016/j.devcel.2018.10.028. (http://www.sciencedirect.com/science/article/pii/S1534580718309171)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:92904
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Feb 2019 17:43
Last Modified:09 Mar 2020 13:19

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