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Structured illumination imaging without grating rotation based on mirror operation on 1D Fourier spectrum

Jin, Xin and Ding, Xuemei and Tan, Jiubin and Yao, Xincheng and Shen, Cheng and Zhou, Xuyang and Tan, Cuimei and Liu, Shutian and Liu, Zhengjun (2019) Structured illumination imaging without grating rotation based on mirror operation on 1D Fourier spectrum. Optics Express, 27 (3). pp. 2016-2028. ISSN 1094-4087. http://resolver.caltech.edu/CaltechAUTHORS:20190221-075930981

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Abstract

Structured illumination microscopy (SIM) is a rapidly developing a super-resolution optical microscopy technique. With SIM, the grating is needed in order to rotate several angles for illuminating the sample in different directions. Multiple rotations reduce the imaging speed and grating rotation angle errors damage the image recovery quality. We introduce mirror transformation on one-dimension (1D) Fourier spectrum to SIM for resolving the problems of low imaging speed and severe impact on image reconstruction quality by grating rotation angle errors. When mirror operation and SIM are combined, the grating is placed at an orientation for obtaining three shadow images. The three shadow images are acquired by CCD at three different phase shift for a direction of grating. Thus, the SIM imaging speed is faster and the effect on image reconstruction quality by grating rotation angle errors is greatly reduced.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1364/oe.27.002016DOIArticle
ORCID:
AuthorORCID
Liu, Shutian0000-0001-9748-0175
Liu, Zhengjun0000-0003-2886-8496
Additional Information:© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. Received 22 Nov 2018; revised 14 Jan 2019; accepted 15 Jan 2019; published 23 Jan 2019. Funding: National Natural Science Foundation of China (Nos. 61575055, 61575053, 11874132); National Institutes of Health (NIH) (Grant ROI EY024628); and Open Fund Project Foundation of Guangdong Provincial Key Laboratory of Modern Geometric and Mechanical Metrology Technology (No. SCMKF201804). The authors express sincere gratitude to Dr. Benquan Wang for his assistance in experimental data.
Funders:
Funding AgencyGrant Number
National Natural Science Foundation of China61575055
National Natural Science Foundation of China61575053
National Natural Science Foundation of China11874132
NIHR01 EY024628
Foundation of Guangdong Provincial Key Laboratory of Modern Geometric and Mechanical Metrology TechnologySCMKF201804
Record Number:CaltechAUTHORS:20190221-075930981
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190221-075930981
Official Citation:Xin Jin, Xuemei Ding, Jiubin Tan, Xincheng Yao, Cheng Shen, Xuyang Zhou, Cuimei Tan, Shutian Liu, and Zhengjun Liu, "Structured illumination imaging without grating rotation based on mirror operation on 1D Fourier spectrum," Opt. Express 27, 2016-2028 (2019)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:93015
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Feb 2019 18:29
Last Modified:21 Feb 2019 18:29

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