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Use of a new strategy to isolate and characterize 436 drosophila cDNA clones corresponding to RNAs detected in adult heads but not in early embryos

Palazzolo, Michael J. and Hyde, David R. and VijayRaghavan, K. and Mecklenburg, Kirk and Benzer, Seymour and Meyerowitz, Elliot (1989) Use of a new strategy to isolate and characterize 436 drosophila cDNA clones corresponding to RNAs detected in adult heads but not in early embryos. Neuron, 3 (4). pp. 527-539. ISSN 0896-6273. http://resolver.caltech.edu/CaltechAUTHORS:20190227-160229748

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Abstract

We describe a new strategy for producing tissue-specific cDNA libraries and subsequently identifying tissue-specific clones. This method was used to screen for cDNA clones corresponding to RNAs expressed in the Drosophila head that cannot be detected in the early embryo. RNA blots were used to assess the spatial and temporal patterns of expression of these RNAs. The ensemble of 436 head-not-embryo clones identified roughly 700 distinct RNAs that are differentially expressed in the Drosophila head. The RNA expression patterns can be classified into five major categories. It is argued that this ensemble of clones represents a large fraction of all genes differentially expressed in the adult head, but not detected in the early embryo. Many of these genes are likely to encode eye- and nervous system-specific products.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0896-6273(89)90211-0DOIArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot0000-0003-4798-5153
Additional Information:© 1989 by Cell Press. Received 31 May 1989, Revised 3 July 1989. We would like to thank Julia Chang, Carol Mayeda, and Michael Whitney for excellent technical assistance. We are also grateful to many colleagues for helpful discussions and to David Anderson, Norman Davidson, and Eric Davidson for comments on the manuscript. Thanks also to Steve Henikoff and Torn Dreesen for suggestions regarding in situ hybridizations to polytene chromosomes. Thanks to Karl Fryxell for the Drosophila major opsin clone. This work was initially supported by NIH grant GM20927 (to E. M. M.) and NSF grant DCB-8409366 (to S. B.) by a grant from the Lucille P. Markey Charitable Trust (jointly to E. M. M. and S. B.), and by NIH program project grant GM40499. M. J. P. was supported by a postdoctoral fellowship from the Helen Hay Whitney Foundation and a Biomedical Scholar award from the Lucille P. Markey Charitable Trust; K. V. was supported by a Procter and Gamble Postdoctoral Fellowship and a Rockefeller Foundation Biotechnology Career Fellowship. D. R. H. and K. M. were supported by NIH postdoctoral fellowships from the National Eye Institute. Michael J. Palazzolo and K. VijayRaghavan are members of Elliot Meyerowitz's group; David R. Hyde and Kirk Mecklenburg are members of Seymour Benzer's group. Both groups contributed equally to this collaborative effort.
Funders:
Funding AgencyGrant Number
NSFGM20927
NSFDCB-8409366
Lucille P. Markey Charitable TrustUNSPECIFIED
NIHGM40499
Helen Hay Whitney FoundationUNSPECIFIED
Procter and GambleUNSPECIFIED
Rockefeller FoundationUNSPECIFIED
NIH Postdoctoral FellowshipUNSPECIFIED
National Eye InstituteUNSPECIFIED
Record Number:CaltechAUTHORS:20190227-160229748
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190227-160229748
Official Citation:Michael J. Palazzolo, David R. Hyde, K. VijayRaghavan, Kirk Mecklenburg, Seymour Benzer, Elliot Meyerowitz, Use of a new strategy to isolate and characterize 436 drosophila cDNA clones corresponding to RNAs detected in adult heads but not in early embryos, Neuron, Volume 3, Issue 4, 1989, Pages 527-539, ISSN 0896-6273, https://doi.org/10.1016/0896-6273(89)90211-0. (http://www.sciencedirect.com/science/article/pii/0896627389902110)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:93323
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Feb 2019 00:12
Last Modified:28 Feb 2019 00:12

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