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Presynaptic Regulation of Neurotransmission in Drosophila by the G Protein-Coupled Receptor Methuselah

Song, Wei and Ranjan, Ravi and Dawson-Scully, Ken and Bronk, Peter and Marin, Leo and Seroude, Laurent and Lin, Yi-Jyun and Nie, Zhiping and Atwood, Harold L. and Benzer, Seymour and Zinsmaier, Konrad E. (2002) Presynaptic Regulation of Neurotransmission in Drosophila by the G Protein-Coupled Receptor Methuselah. Neuron, 36 (1). pp. 105-119. ISSN 0896-6273. doi:10.1016/s0896-6273(02)00932-7. https://resolver.caltech.edu/CaltechAUTHORS:20190228-115827198

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Abstract

Regulation of synaptic strength is essential for neuronal information processing, but the molecular mechanisms that control changes in neuroexocytosis are only partially known. Here we show that the putative G protein-coupled receptor Methuselah (Mth) is required in the presynaptic motor neuron to acutely upregulate neurotransmitter exocytosis at larval Drosophila NMJs. Mutations in the mth gene reduce evoked neurotransmitter release by ∼50%, and decrease synaptic area and the density of docked and clustered vesicles. Pre- but not postsynaptic expression of normal Mth restored normal release in mth mutants. Conditional expression of Mth restored normal release and normal vesicle docking and clustering but not the reduced size of synaptic sites, suggesting that Mth acutely adjusts vesicle trafficking to synaptic sites.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0896-6273(02)00932-7DOIArticle
Additional Information:© 2002 Cell Press. Under an Elsevier user license. Received 19 December 2001, Revised 25 July 2002, Available online 7 October 2002. We thank Dr. Corey Goodman for Fas-II antibodies and the CD8-GFP-Sh strain and Drs. Alexander Petrenko and Yuri Ushkaryov for purified latro- and latroinsectotoxins. We thank Sheng Wang for assistance with electron microscopy. We thank Drs. Peter Sterling, Mike Nusbaum, Brian Salzberg, and Phil Haydon for critical comments on the manuscript and Dr. Irwin Levitan for his extraordinary support during the last stages of this study. This work has been in part supported by grants to K.E.Z. from the National Science Foundation (IBN-9604889) and the National Institutes of Health (RO1NS38274), by a National Research Service Award to P.B. from NIMH (MH12611F31), by a Provost Award to W.S., by grants to S.B. from the National Science Foundation (MCB9907939), the National Institutes of Health (AG16630 and EY09278), and the Ellison Foundation, and grants to H.L.A. from the Natural Sciences and Engineering Research Council of Canada (NSERC).
Funders:
Funding AgencyGrant Number
NSFIBN-9604889
NIHRO1NS38274
NIHMH12611F31
University of PennsylvaniaUNSPECIFIED
NSFMCB-9907939
NIHAG16630
NIHEY09278
Ellison Medical FoundationUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Issue or Number:1
DOI:10.1016/s0896-6273(02)00932-7
Record Number:CaltechAUTHORS:20190228-115827198
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190228-115827198
Official Citation:Wei Song, Ravi Ranjan, Ken Dawson-Scully, Peter Bronk, Leo Marin, Laurent Seroude, Yi-Jyun Lin, Zhiping Nie, Harold L. Atwood, Seymour Benzer, Konrad E. Zinsmaier, Presynaptic Regulation of Neurotransmission in Drosophila by the G Protein-Coupled Receptor Methuselah, Neuron, Volume 36, Issue 1, 2002, Pages 105-119, ISSN 0896-6273, https://doi.org/10.1016/S0896-6273(02)00932-7. (http://www.sciencedirect.com/science/article/pii/S0896627302009327)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:93344
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:28 Feb 2019 21:23
Last Modified:16 Nov 2021 16:57

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