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Presynaptic dysfunction in drosophila csp mutants

Umbach, Joy A. and Zinsmaier, Konrad E. and Eberle, Kai K. and Buchner, Erich and Benzer, Seymour and Gundersen, Cameron B. (1994) Presynaptic dysfunction in drosophila csp mutants. Neuron, 13 (4). pp. 899-907. ISSN 0896-6273. https://resolver.caltech.edu/CaltechAUTHORS:20190228-115828203

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Abstract

Cysteine string proteins are synapse-specific proteins. In Drosophila, csp deletion mutants exhibit temperature-sensitive paralysis and early death. Here, we report that neuromuscular transmission is impaired presynaptically in these csp mutant larvae. At 22°C, evoked transmitter release is depressed relative to wild type and rescued controls, and high frequency stimulation of the nerve leads to sporadic failures. At 30°C, stimulus-evoked responses decline gradually before failing completely. When the temperature is returned to 22°C, evoked responses recover. Spontaneous release events persist at both 22°C and 30°C. Since nerve conduction and postsynaptic sensitivity are unaffected, these data indicate that csp mutations disrupt depolarization-secretion coupling. This disruption explains the cellular basis of the temperature-sensitive paralysis of these organisms.


Item Type:Article
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https://doi.org/10.1016/0896-6273(94)90255-0DOIArticle
Additional Information:© 1994 Cell Press. Received 26 May 1994, Revised 20 July 1994. We thank Y. Kidokoro and A. Grinnell for helpful discussions, T. Schwarz and H. Atwood for work in press, and A. Chang and M. Kreman for assistance with figures. Funded by grants from National Institutes of Health J. A. U. and S. B.), National Science Foundation (S. B.), DFG (K. E. Z. and E. B.), and the J. G. Boswell Foundation (S. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement”in accordance with 18 USC Section 1734 solely to indicate this fact.
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NIHUNSPECIFIED
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Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
James G. Boswell FoundationUNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20190228-115828203
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190228-115828203
Official Citation:Joy A. Umbach, Konrad E. Zinsmaier, Kai K. Eberle, Erich Buchner, Seymour Benzer, Cameron B. Gundersen, Presynaptic dysfunction in drosophila csp mutants, Neuron, Volume 13, Issue 4, 1994, Pages 899-907, ISSN 0896-6273, https://doi.org/10.1016/0896-6273(94)90255-0. (http://www.sciencedirect.com/science/article/pii/0896627394902550)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:93352
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:28 Feb 2019 20:51
Last Modified:03 Oct 2019 20:53

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