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Subcellular localization of transcripts in Drosophila photoreceptor neurons: chaoptic mutants have an aberrant distribution

Pollock, John A. and Ellisman, Mark H. and Benzer, Seymour (1990) Subcellular localization of transcripts in Drosophila photoreceptor neurons: chaoptic mutants have an aberrant distribution. Genes and Development, 4 (5). pp. 806-821. ISSN 0890-9369. https://resolver.caltech.edu/CaltechAUTHORS:20190228-155954991

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Abstract

Photoreceptor neurons in the Drosophila retina are long (100 mu) and narrow, providing a system for the study of the intracellular distribution of transcripts and proteins. The chaoptic gene is expressed exclusively in photoreceptor neurons, and mutations of the gene result in reduced developmental competence of cells to generate normal rhabdomeric membranes. The mutant protein exhibited altered distribution both in developing and adult photoreceptor neurons. Furthermore, the transcript distribution in mutants was altered, decreasing with distance from the nucleus, instead of the normal uniform distribution throughout the cell soma. The deficit of transcript concentration correlated with the severity of developmental defect in rhabdomere formation along the cell. In contrast, the distribution of the opsin transcript was not affected by the chaoptic mutation. To observe RNA localization at the ultrastructural level, a high-resolution, electron microscopic in situ hybridization protocol was developed. The results indicate that the normal chaoptic transcript is present on the rough endoplasmic reticulum, which may be a vehicle for specific transcript distribution.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://dx.doi.org/10.1101/gad.4.5.806DOIArticle
Additional Information:©1990 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). Received November 3, 1989; revised version accepted March 2, 1990. We express our thanks to Larry Zipursky, Rosemary Reinke, Dave Van Vactor, and their co-workers for their help and generosity. We also thank Charles Zuker for the ninaE clone and Herschel Mitchell for sharing his wealth of knowledge. We are indebted to Nancy Bonini, Utpal Banerjee, and Susan Celnicker, whose comments on this work and this paper were invaluable. Ultracryomicrotomy was performed by Thomas Deerinck. Expert technical assistance was provided by Marika Anderson, Julia Chang, Eveline Eichenberger, Jenny Mao, and Rosalind Young. George Wray, Paul Connaly, and Mary Morphew provided technical support for the HVEM work in Boulder, Colorado. This work was supported by grants from the National Institutes of Health (NIH) (EY05863 and EY08334-01 to J.A.P.; NS14718, NS26739, and RR04050 to M.H.E.; and P01-GM40499 to S.B.) and from the National Science Foundation (BNS 88-10098 to M.H.E and DCB-8409366 to S.B.), as well as by a developmental biology grant from the Lucille P. Markey Charitable Trust to J.A.P. and S.B. The HVEM facilities in Boulder, Colorado, were supported by the NIH Division of Research Resources (RR 00592); J.A.P. was the recipient of an HVEM Scholar Award from this facility.
Funders:
Funding AgencyGrant Number
NIHEY05863
NIHEY08334-01
NIHNS14718
NIHNS26739
NIHRR04050
NIHP01-GM40499
NSFBNS 88-10098
NSFDCB-8409366
Lucille P. Markey Charitable TrustUNSPECIFIED
NIHRR00592
NIH Postdoctoral FellowshipUNSPECIFIED
Subject Keywords:Retina; photoreceptors; EM in situ hybridization; MAb24B10
Issue or Number:5
Record Number:CaltechAUTHORS:20190228-155954991
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190228-155954991
Official Citation:Subcellular localization of transcripts in Drosophila photoreceptor neurons: chaoptic mutants have an aberrant distribution. J A Pollock, M H Ellisman, and S Benzer Genes Dev. May 1990 4: 806-821; doi:10.1101/gad.4.5.806
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:93367
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:01 Mar 2019 17:39
Last Modified:03 Oct 2019 20:53

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