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On the role of soluble ribonucleic acid in coding for amino acids

Chapeville, Francois and Lipmann, Fritz and von Ehrenstein, Günter and Weisblum, Bernard and Ray, William J., Jr. and Benzer, Seymour (1962) On the role of soluble ribonucleic acid in coding for amino acids. Proceedings of the National Academy of Sciences of the United States of America, 48 (6). pp. 1086-1092. ISSN 0027-8424. PMCID PMC220908. doi:10.1073/pnas.48.6.1086.

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In protein synthesis, each amino acid is first joined specifically with a corresponding sRNA through the mediation of an activating enzyme. These aminoacyl-sRNA's, by reaction with a ribosomal preparation, form proteins with specific amino acid sequences. According to the "'adaptor" hypothesis of Crick and Hoagland, the position of a particular amino acid would be determined not by the amino acid itself, but by hydrogen bonding between the RNA template and a complementary nucleotide sequence in the sRNA carrying the amino acid. The experiment described in this paper was designed as a direct test of the adaptor hypothesis, by attaching an amino acid to its normal sRNA and then, without breaking the bond, converting the amino acid to another one of the natural amino acids. It is then possible to determine whether the coding properties of this hybrid are determined by the sRNA or the amino acid. We have made use of the fact that cysteine can be altered by reductive desulfhydration with Raney Nickel to alanine.

Item Type:Article
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URLURL TypeDescription CentralArticle
Additional Information:© 1962 National Academy of Sciences. Communicated April 25, 1962. Supported by grants from the National Science Foundation and from the National Cancer Institute, National Institutes of Health, U.S. Public Health Service.
Funding AgencyGrant Number
National Cancer InstituteUNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC220908
Record Number:CaltechAUTHORS:20190301-083121900
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Official Citation:ON THE ROLE OF SOLUBLE RIBONUCLEIC ACID IN CODING FOR AMINO ACIDS Francois Chapeville, Fritz Lipmann, Günter von Ehrenstein, Bernard Weisblum, William J. Ray, Seymour Benzer Proceedings of the National Academy of Sciences Jun 1962, 48 (6) 1086-1092; DOI: 10.1073/pnas.48.6.1086
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:93380
Deposited By: George Porter
Deposited On:01 Mar 2019 18:24
Last Modified:16 Nov 2021 16:57

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