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Cytotoxic and apoptotic inducing activity of Amoora rohituka leaf extracts in human breast cancer cells

Singh, Rajesh Kumar and Ranjan, Amit and Srivastava, Akhileshwar Kumar and Singh, Monika and Shukla, Anil Kumar and Atri, Neelam and Mishra, Anurag and Singh, Anil Kumar and Singh, Santosh Kumar (2020) Cytotoxic and apoptotic inducing activity of Amoora rohituka leaf extracts in human breast cancer cells. Journal of Ayurveda and Integrative Medicine, 11 (4). pp. 383-390. ISSN 0975-9476. PMCID PMC7772503. doi:10.1016/j.jaim.2018.12.005.

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Background: Amoora rohituka is described in Ayurveda, an Indian traditional system of medicine for management of disorders of blood, diseases of eye, helminthiasis disease, ulcer, liver disorders and splenomegaly. However, the leaves were not reported to have anticancer properties till date. Objective: This study was carried out to evaluate the cytotoxic potential of leaf extracts of Amoora rohituka. Materials and methods: The leaves powder was macerated in petroleum ether, ethyl acetate and methanol and evaluated their anticancer activities in vitro. The phytochemical constituents of the active (ethyl acetate) extract were screened by FTIR analysis and phytochemical screening methods. Results: The ethyl acetate extract (RLEA) showed the presence of alkaloids, flavonoids, steroids, tannins, saponins and terpenoids. The RLEA exhibited high cytotoxic effect against human breast cancer cells, MCF-7 (IC50 = 9.81 μg/mL) and induced apoptosis by altering nuclear morphology and DNA laddering. Wound healing assays explained the potency of extract to decrease the cell migration. Conclusion: The extract of Amoora rohituka leaves exhibited anticancer activity with less toxicity and it could be used for development of alternative drugs in the treatment of human breast cancer.

Item Type:Article
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Singh, Rajesh Kumar0000-0003-3473-8209
Additional Information:© 2019 The Authors. Published by Elsevier B.V. on behalf of Institute of Transdisciplinary Health Sciences and Technology and World Ayurveda Foundation. This is an open access article under the CC BY-NC-ND license ( Received 13 August 2018, Revised 8 December 2018, Accepted 22 December 2018, Available online 4 March 2019. We are thankful to the Centre of Experimental Medicine & Surgery (CEMS), Institute of Medical Sciences, Banaras Hindu University, Varanasi, India for instrument facilities. Source(s) of funding: The author Rajesh Kumar Singh is grateful to BHU and UGC for financial supports as fellowships (No. R/Dev/Scholarship (SRF-JRF)/Ayurveda/57032. Conflict of interest: None.
Funding AgencyGrant Number
Banaras Hindu UniversityUNSPECIFIED
University Grants Commission57032
Subject Keywords:Traditional medicine; Breast cancer; FTIR; HPTLC; Apoptosis; Cell migration
Issue or Number:4
PubMed Central ID:PMC7772503
Record Number:CaltechAUTHORS:20190304-134833117
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Official Citation:Rajesh Kumar Singh, Amit Ranjan, Akhileshwar Kumar Srivastava, Monika Singh, Anil Kumar Shukla, Neelam Atri, Anurag Mishra, Anil Kumar Singh, Santosh Kumar Singh, Cytotoxic and apoptotic inducing activity of Amoora rohituka leaf extracts in human breast cancer cells, Journal of Ayurveda and Integrative Medicine, Volume 11, Issue 4, 2020, Pages 383-390, ISSN 0975-9476, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:93445
Deposited By: Tony Diaz
Deposited On:04 Mar 2019 22:00
Last Modified:16 Nov 2021 16:57

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