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Hybrid-scanning optical-resolution photoacoustic microscopy for in vivo vasculature imaging

Rao, Bin and Li, Li and Maslov, Konstantin and Wang, Lihong (2010) Hybrid-scanning optical-resolution photoacoustic microscopy for in vivo vasculature imaging. Optics Letters, 35 (10). pp. 1521-1523. ISSN 0146-9592. http://resolver.caltech.edu/CaltechAUTHORS:20190305-153255524

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Abstract

Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, is complementary to other optical microscopy modalities such as optical confocal microscopy, optical coherence tomography, and multiphoton microscopy. A hybrid optical–mechanical scanning configuration increases the imaging speed of OR-PAM significantly, facilitating many demanding biomedical applications. With a high-pulse-repetition-rate laser, the hybrid-scanning OR-PAM can acquire one-dimensional depth-resolved images (A-lines) at 5 kHz and two-dimensional B-scan images containing 800 A-lines at 6.25 Hz. We demonstrated in vivo in a mouse three-dimensional imaging of the iris vasculature in 128 s for an 800×800×200 data set and of the ear vasculature in 256 s for an 800×1600×200 data set.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1364/ol.35.001521DOIArticle
ORCID:
AuthorORCID
Rao, Bin0000-0001-6494-3110
Wang, Lihong0000-0001-9783-4383
Additional Information:© 2010 Optical Society of America. Received February 11, 2010; revised March 24, 2010; accepted March 31, 2010; posted April 6, 2010 (Doc. ID 124078); published May 5, 2010. The research was supported by the National Institutes of Health (NIH) grants R01 EB000712, EB000712A2S1, R01 EB00071207S2, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. L.W. has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHEB000712A2S1
NIHR01 EB00071207S2
NIHR01 EB008085
NIHR01 CA113453901
NIHU54 CA136398
NIH5P60 DK02057933
Classification Code:OCIS codes: 170.3880, 170.5120, 180.5810, 110.5120, 110.0180
Record Number:CaltechAUTHORS:20190305-153255524
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190305-153255524
Official Citation:Bin Rao, Li Li, Konstantin Maslov, and Lihong Wang, "Hybrid-scanning optical-resolution photoacoustic microscopy for in vivo vasculature imaging," Opt. Lett. 35, 1521-1523 (2010)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:93553
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:06 Mar 2019 00:11
Last Modified:06 Mar 2019 00:11

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