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In Vivo Label-free Functional Photoacoustic Monitoring of Ischemic Reperfusion

Bi, Renzhe and Dinish, U. S. and Goh, Chi Ching and Imai, Toru and Moothanchery, Mohesh and Li, Xiuting and Kim, Jin Young and Jeon, Seungwan and Pu, Yang and Kim, Chulhong and Ng, Lai Guan and Wang, Lihong V. and Olivo, Malini (2019) In Vivo Label-free Functional Photoacoustic Monitoring of Ischemic Reperfusion. Journal of Biophotonics, 12 (7). Art. No. e201800454. ISSN 1864-063X.

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Pressure ulcer (PU) formation is a common problem among patients confined to bed or restricted to wheelchairs. The ulcer forms when the affected skin and underlying tissues go through repeated cycles of ischemia and reperfusion, leading to inflammation. This theory is evident by intravital imaging studies performed in immune cell‐specific, fluorescent reporter mouse skin with induced ischemia‐reperfusion (I‐R) injuries. However, traditional confocal or multi‐photon microscopy cannot accurately monitor the progression of vascular reperfusion by contrast agents, which leaks into the interstitium under inflammatory conditions. Here, we develop a dual‐wavelength micro electro mechanical system (MEMS) scanning based optical resolution photoacoustic microscopy (OR‐PAM) system for continuous label‐free functional imaging of vascular reperfusion in an IR mouse model. This MEMS‐OR‐PAM system provides fast scanning speed for concurrent dual‐wavelength imaging, which enables continuous monitoring of the reperfusion process. During reperfusion, the revascularization of blood vessels and the oxygen saturation (sO_2) changes in both arteries and Illustration of the vascular reperfusion of the ear skin. veins are recorded, from which the local oxygen extraction ratios of the ischemic tissue and the unaffected tissue can be quantified. Our MEMS‐OR‐PAM system provides novel perspectives to understand the I‐R injuries. It solves the problem of dynamic label‐free functional monitoring of the vascular reperfusion at high spatial resolution.

Item Type:Article
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URLURL TypeDescription
Imai, Toru0000-0002-9497-8549
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2019 Wiley. Accepted manuscript online: 13 March 2019; Manuscript accepted: 03 March 2019; Manuscript received: 07 December 2018.
Subject Keywords:photoacoustic imaging; ischemia reperfusion; skin; image processing
Issue or Number:7
Record Number:CaltechAUTHORS:20190313-094535694
Persistent URL:
Official Citation:Bi, R, Dinish, US, Goh, CC, et al. In vivo label‐free functional photoacoustic monitoring of ischemic reperfusion. J. Biophotonics. 2019; 12:e201800454.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:93764
Deposited By: Tony Diaz
Deposited On:13 Mar 2019 16:59
Last Modified:03 Oct 2019 20:57

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