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Prandiology of Drosophila and the CAFE assay

Ja, William W. and Carvalho, Gil B. and Mak, Elizabeth M. and de la Rosa, Noelle N. and Fang, Annie Y. and Liong, Jonathan C. and Brummel, Ted and Benzer, Seymour (2007) Prandiology of Drosophila and the CAFE assay. Proceedings of the National Academy of Sciences of the United States of America, 104 (20). pp. 8253-8256. ISSN 0027-8424. PMCID PMC1899109. https://resolver.caltech.edu/CaltechAUTHORS:JAWpnas07

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Abstract

Studies of feeding behavior in genetically tractable invertebrate model systems have been limited by the lack of proper methodology. We introduce the Capillary Feeder (CAFE), a method allowing precise, real-time measurement of ingestion by individual or grouped fruit flies on the scale of minutes to days. Using this technique, we conducted the first quantitative analysis of prandial behavior in Drosophila melanogaster. Our results allow the dissection of feeding into discrete bouts of ingestion, defining two separate parameters, meal volume and frequency, that can be uncoupled and thus are likely to be independently regulated. In addition, our long-term measurements show that flies can ingest as much as 1.7x their body mass over 24 h. Besides the study of appetite, the CAFE can be used to monitor oral drug delivery. As an illustration, we used the CAFE to test the effects of dietary supplementation with two compounds, paraquat and ethanol, on food ingestion and preference. Paraquat, a prooxidant widely used in stress tests, had a strong anorexigenic effect. In contrast, in a feeding preference assay, ethanol-laced food, but not ethanol by itself, acted as an attractant.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1899109/PubMed CentralArticle
https://doi.org/10.1073/pnas.0702726104DOIUNSPECIFIED
https://doi.org/10.1073/pnas.0702726104DOIUNSPECIFIED
Additional Information:© 2007 by the National Academy of Sciences. Contributed by Seymour Benzer, March 26, 2007 (received for review March 14, 2007). Published online before print May 9, 2007, 10.1073/pnas.0702726104 We thank current and past members of the S.B. laboratory for helpful discussions. This work was supported by a postdoctoral fellowship from the John Douglas French Alzheimer's Foundation (to W.W.J.); a Lawrence L. and Audrey W. Ferguson Fellowship (to G.B.C.); a Nellie Bergen and Adrian Foster Tillotson Summer Undergraduate Research Fellowship from the California Institute of Technology (to J.C.L.); National Institutes of Health Grants AG16630, AG24366, and DK070154; National Science Foundation Grant MCB-0418479; and a grant from the Ellison Foundation (to S.B.). Author contributions: W.W.J. and G.B.C. contributed equally to this work; W.W.J., G.B.C., E.M.M., N.N.d.l.R., A.Y.F., and T.B. designed research; W.W.J., G.B.C., E.M.M., A.Y.F., and J.C.L. performed research; W.W.J. contributed new reagents/analytical tools; and W.W.J., G.B.C., E.M.M., N.N.d.l.R., and S.B. wrote the paper. The authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
John Douglas French Alzheimer’s FoundationUNSPECIFIED
Lawrence L. and Audrey W. Ferguson FellowshipUNSPECIFIED
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
NIHAG16630
NIHAG24366
NIHDK070154
NSFMCB-0418479
Ellison Medical FoundationUNSPECIFIED
Subject Keywords:appetite; feeding; ingestion; preference
Issue or Number:20
PubMed Central ID:PMC1899109
Record Number:CaltechAUTHORS:JAWpnas07
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:JAWpnas07
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9386
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:17 Dec 2007
Last Modified:02 Oct 2019 23:59

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