Design and performance of in vitro transcription rate regulatory circuits

Franco, Elisa and Murray, Richard M. (2008) Design and performance of in vitro transcription rate regulatory circuits. In: 47th IEEE Conference on Decision and Control. IEEE , Piscataway, NJ, pp. 161-166. ISBN 978-1-4244-3123-6. https://resolver.caltech.edu/CaltechAUTHORS:20190322-143454266

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Abstract

This paper proposes a synthetic in vitro circuit that aims at regulating the rate of RNA transcription through positive feedback interactions. This design is dual to a previously synthesized transcriptional rate regulator based on self-repression. Two DNA templates are designed to interact through their transcripts, creating cross activating feedback loops that will equate their transcription rates at steady state. A mathematical model is developed for this circuit, consisting of a set of ODEs derived from the mass action laws and Michaelis-Menten kinetics involving all the present chemical species. This circuit is then compared to its regulatory counterpart based on negative feedback. A global sensitivity analysis reveals the fundamental features of the two designs by evaluating their equilibrium response to changes in the most crucial parameters of the system.

Item Type:

Book Section

Related URLs:

URL	URL Type	Description
https://doi.org/10.1109/CDC.2008.4739253	DOI	Article

ORCID:

Author	ORCID
Franco, Elisa	0000-0003-1103-2668
Murray, Richard M.	0000-0002-5785-7481

Additional Information:

© 2008 IEEE. Research supported in part by the Institute for Collaborative Biotechnologies through grant DAAD19-03-D-0004 from the U.S. Army Research Office. The authors would like to thank Erik Winfree, Jongmin Kim, Per-Ola Forsberg and all the members of the DNA and Natural Algorithms group at Caltech for their helpful advise during the development of this project.

Funders:

Funding Agency	Grant Number
Army Research Office (ARO)	DAAD19-03-D-0004

DOI:

10.1109/CDC.2008.4739253

Record Number:

CaltechAUTHORS:20190322-143454266

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20190322-143454266

Official Citation:

E. Franco and R. M. Murray, "Design and performance of in vitro transcription rate regulatory circuits," 2008 47th IEEE Conference on Decision and Control, Cancun, 2008, pp. 161-166. doi: 10.1109/CDC.2008.4739253

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No commercial reproduction, distribution, display or performance rights in this work are provided.

ID Code:

94071

Collection:

CaltechAUTHORS

Deposited By:

Tony Diaz

Deposited On:

22 Mar 2019 23:09

Last Modified:

16 Nov 2021 17:02

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