A Caltech Library Service

Design and performance of in vitro transcription rate regulatory circuits

Franco, Elisa and Murray, Richard M. (2008) Design and performance of in vitro transcription rate regulatory circuits. In: 47th IEEE Conference on Decision and Control. IEEE , Piscataway, NJ, pp. 161-166. ISBN 978-1-4244-3123-6.

[img] PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


This paper proposes a synthetic in vitro circuit that aims at regulating the rate of RNA transcription through positive feedback interactions. This design is dual to a previously synthesized transcriptional rate regulator based on self-repression. Two DNA templates are designed to interact through their transcripts, creating cross activating feedback loops that will equate their transcription rates at steady state. A mathematical model is developed for this circuit, consisting of a set of ODEs derived from the mass action laws and Michaelis-Menten kinetics involving all the present chemical species. This circuit is then compared to its regulatory counterpart based on negative feedback. A global sensitivity analysis reveals the fundamental features of the two designs by evaluating their equilibrium response to changes in the most crucial parameters of the system.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Franco, Elisa0000-0003-1103-2668
Murray, Richard M.0000-0002-5785-7481
Additional Information:© 2008 IEEE. Research supported in part by the Institute for Collaborative Biotechnologies through grant DAAD19-03-D-0004 from the U.S. Army Research Office. The authors would like to thank Erik Winfree, Jongmin Kim, Per-Ola Forsberg and all the members of the DNA and Natural Algorithms group at Caltech for their helpful advise during the development of this project.
Funding AgencyGrant Number
Army Research Office (ARO)DAAD19-03-D-0004
Record Number:CaltechAUTHORS:20190322-143454266
Persistent URL:
Official Citation:E. Franco and R. M. Murray, "Design and performance of in vitro transcription rate regulatory circuits," 2008 47th IEEE Conference on Decision and Control, Cancun, 2008, pp. 161-166. doi: 10.1109/CDC.2008.4739253
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94071
Deposited By: Tony Diaz
Deposited On:22 Mar 2019 23:09
Last Modified:16 Nov 2021 17:02

Repository Staff Only: item control page